reporter assay

[Clinical and Translational Oncology] The location of LINC00261 in PC cells was identified by RNA fluorescence in situ hybridization (RNA-FISH).

[Frontiers in Bioscience-Landmark] Using flow cytometry and western blot assays, the authors have demonstrated that programmed cell death ligand 1 expression is higher in endometrial cancer stem-like cell derived from endometrial cancer than in nonstem-like cancer cells.

[Cardiovascular Drugs and Therapy] Oxidized low-density lipoprotein was used to treat human umbilical vein endothelial cells to construct a cell injury model. The expression level of circCHMP5, miR-532-5p, and Rho-associated protein kinase 2 was measured using quantitative real-time PCR.

[Cell Death Discovery] Gene expressions of XR_596701 and miR-344b-5p were detected by quantitative real-time polymerase chain reaction in intermittent hypoxia-induced H9c2 cells.

[Laboratory Investigation] Researchers revealed that hypoxia/reoxygenation (H/R) treated human cardiac myocytes showed a significantly decreased miR-185-5p expression and increased expression of CDK6 and ROR.

[Experimental Hematology & Oncology] In vitro mammary spheroid formation, flow cytometry assay on CD24−/CD44+ sub-population, ALDH activity detection, cell viability assay and western blot analysis, and in vivo tumor-initiating analysis were performed to examine the effects of Tanshinone IIA on the stemness of breast cancer cells.