reporter assay

[Circulation Journal] The expression of nuclear paraspeckle assembly transcript 1, miR-30d-5p and A disintegrin and metalloprotease 10 was measured by qRT-PCR and western blot. Functional experiments were conducted by using a CCK-8 assay, EDU assay, flow cytometry, western blot, ELISA, and commercial kits.

[Molecular Medicine Reports] Scientists investigated the involvement of Interferon regulatory factor (IRF) 2 in myocardial infarction (MI) and the underlying mechanism of IRF2 in pyroptosis. To mimic MI, ligation of the left anterior descending coronary artery was performed to establish an in vivo mouse model and rat cardiomyocytes H9c2 cells were cultured under hypoxic conditions to establish an in vitro model.

[Journal of Surgical Oncology] The authors explored the expression level of miR-214-5p in prostate cancer and made a preliminary study of its molecular mechanism in the development of prostate cancer to provide effective new strategies for the treatment of prostate cancer.

[andrology] CCK-8, colony formation, transwell, tube formation and flow cytometry assays were applied to assess cell proliferation, motility, angiogenesis, cell cycle distribution and apoptosis.

[ACS Omega] Researchers investigate the function of miR-199 on gemcitabine-resistance in pancreatic cancer, as well as the underlying mechanism.

[Biomedical Journal] Scientists probed into the role and the underlying regulatory mechanism of apoptosis-inducing factor mitochondria associated 1 (AIFM1) in myocardial injury.