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single-cell RNA sequencing

Single-Cell eQTL Mapping Identifies Cell Type-Specific Genetic Control of Autoimmune Disease

[Science] Researchers developed a framework for the classification of individual cells, and by combining single-cell RNA sequencing data with genotype data, they mapped the genetic effects on gene expression in each of 14 immune cell types and identified 26,597 independent cis–expression quantitative trait loci (eQTLs).

Single-Cell Transcriptional Diversity of Neonatal Umbilical Cord Blood Immune Cells Reveals Neonatal Immune Tolerance

[Biochemical and Biophysical Research Communications] Scientists applied single-cell RNA sequencing and single-cell sequencing assay for transposase-accessible chromatin to systematically profile umbilical cord blood nucleated cells and peripheral blood mononuclear cells to identify their composition and differentially expressed genes.

Specification of Fetal Liver Endothelial Progenitors to Functional Zonated Adult Sinusoids Requires C-maf Induction

[Cell Stem Cell] Scientists studied heterogeneity of hepatic vascular cells during mouse development through functional and single-cell RNA-sequencing.

SARS-CoV-2 Antigen Exposure History Shapes Phenotypes and Specificity of Memory CD8+ T Cells

[Nature Immunology] The authors utilize major histocompatibility complex multimers with single-cell RNA sequencing to profile SARS-CoV-2-responsive T cells ex vivo from humans with one, two or three antigen exposures, including vaccination, primary infection and breakthrough infection.

Defining the Early Stages of Intestinal Colonization by Whipworms

[Nature Communications] Scientists investigated early infection events using both Trichuris muris infections of mice and murine caecaloids, the first in vitro system for whipworm infection and organoid model for live helminths.

IL-22 Plays a Dual Role in the Amniotic Cavity: Tissue Injury and Host Defense against Microbes in Preterm Labor

[Journal of Immunology] Scientists showed that IL-22+ T cells coexpressing retinoic acid–related orphan receptor γt were enriched at the human maternal-fetal interface of women with preterm labor and birth, which was confirmed by in silico analysis of single-cell RNA sequencing data.

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