Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition

Using a high-throughput approach that combines enteroid monolayers and quantitative imaging, scientists identified conditions that enrich for specific cell types as well as interactions between pathways.
[Developmental Cell]
Sanman, L. E., Chen, I. W., Bieber, J. M., Steri, V., Trentesaux, C., Hann, B., Klein, O. D., Wu, L. F., & Altschuler, S. J. (2021). Transit-Amplifying Cells Coordinate Changes in Intestinal Epithelial Cell-Type Composition. Developmental Cell, 0(0). https://doi.org/10.1016/j.devcel.2020.12.020 Cite
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Ethanol Exposure Drives Colon Location Specific Cell Composition Changes in a Normal Colon Crypt 3D Organoid Model

Scientists employed RNA-sequencing to asses transcriptomic response to ethanol exposure in 3D organoid lines derived from healthy colon. Paired regression analysis identified 2,162 differentially expressed genes in response to ethanol.
[Scientific Reports]
Devall, M., Plummer, S. J., Bryant, J., Jennelle, L. T., Eaton, S., Dampier, C. H., Huyghe, J. R., Peters, U., Powell, S. M., & Casey, G. (2021). Ethanol exposure drives colon location specific cell composition changes in a normal colon crypt 3D organoid model. Scientific Reports, 11(1), 432. https://doi.org/10.1038/s41598-020-80240-1 Cite
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AP-1 and TGFß Cooperativity Drives Non-Canonical Hedgehog Signaling in Resistant Basal Cell Carcinoma

The authors used single cell RNA-sequencing of patient tumors to identify three prognostic surface markers (LYPD3, TACSTD2, and LY6D) which correlated with myocardin-related transcription factor (nMRTF) and resistance to Smoothened inhibitors. The nMRTF cell state resembled transit-amplifying cells of the hair follicle matrix, with AP-1 and TGFß cooperativity driving nMRTF activation.
[Nature Communications]
Yao, C. D., Haensel, D., Gaddam, S., Patel, T., Atwood, S. X., Sarin, K. Y., Whitson, R. J., McKellar, S., Shankar, G., Aasi, S., Rieger, K., & Oro, A. E. (2020). AP-1 and TGFß cooperativity drives non-canonical Hedgehog signaling in resistant basal cell carcinoma. Nature Communications, 11(1), 5079. https://doi.org/10.1038/s41467-020-18762-5 Cite
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