Tag Archives: type-publication

A Highly Multiplexed Droplet Digital PCR Assay to Measure the Intact HIV-1 Proviral Reservoir

Scientists designed two triplex droplet digital PCR assays, each with two unique targets and one in common, and normalize the results to PCR-based T cell counts.
[Cell Reports Medicine]
Levy, C. N., Hughes, S. M., Roychoudhury, P., Reeves, D. B., Amstuz, C., Zhu, H., Huang, M.-L., Wei, Y., Bull, M. E., Cassidy, N. A. J., McClure, J., Frenkel, L. M., Stone, M., Bakkour, S., Wonderlich, E. R., Busch, M. P., Deeks, S. G., Schiffer, J. T., Coombs, R. W., … Hladik, F. (2021). A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir. Cell Reports Medicine, 0(0). https://doi.org/10.1016/j.xcrm.2021.100243 Cite
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Neutrophil Subtypes Shape HIV-Specific CD8 T-cell Responses after Vaccinia Virus Infection

Scientists found that these neutrophil subtypes showed distinct migratory and motility patterns and different ability to interact with CD8 T cells in the spleen following vaccinia virus infection.
[npj Vaccines]
Di Pilato, M., Palomino-Segura, M., Mejías-Pérez, E., Gómez, C. E., Rubio-Ponce, A., D’Antuono, R., Pizzagalli, D. U., Pérez, P., Kfuri-Rubens, R., Benguría, A., Dopazo, A., Ballesteros, I., Sorzano, C. O. S., Hidalgo, A., Esteban, M., & Gonzalez, S. F. (2021). Neutrophil subtypes shape HIV-specific CD8 T-cell responses after vaccinia virus infection. Npj Vaccines, 6(1), 1–11. https://doi.org/10.1038/s41541-021-00314-7 Cite
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Control of IFN-I Responses by the Aminopeptidase IRAP in Neonatal C57BL/6 Alveolar Macrophages during RSV Infection

Neonatal C57BL/6 alveolar macrophages mobilized very weakly the IFN-I pathway upon Respiratory Syncytial Virus (RSV) infection in vitro and failed to restrain virus replication.
[Mucosal Immunology]
Drajac, C., Laubreton, D., Marquant, Q., Chottin, C., Ferret, C., Bouguyon, E., Schwartz-Cornil, I., Saveanu, L., Riffault, S., & Descamps, D. (2021). Control of IFN-I responses by the aminopeptidase IRAP in neonatal C57BL/6 alveolar macrophages during RSV infection. Mucosal Immunology, 1–14. https://doi.org/10.1038/s41385-021-00402-w Cite
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Human Airway Cells Prevent SARS-CoV-2 Multibasic Cleavage Site Cell Culture Adaptation

Researchers report that propagating SARS-CoV-2 on the human airway cell line Calu-3 prevented cell culture adaptations in the multibasic cleavage sites. Similar results were obtained using a human airway organoid-based culture system for SARS-CoV-2 propagation.
[eLife]
Lamers, M. M., Mykytyn, A. Z., Breugem, T. I., Wang, Y., Wu, D. C., Riesebosch, S., van den Doel, P. B., Schipper, D., Bestebroer, T., Wu, N. C., & Haagmans, B. L. (2021). Human airway cells prevent SARS-CoV-2 multibasic cleavage site cell culture adaptation. ELife, 10, e66815. https://doi.org/10.7554/eLife.66815 Cite
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Adhesion Receptor Adgrg2/Gpr64 Is In The Gi-Tract Selectively Expressed In Mature Intestinal Tuft Cells

Time-lapse studies of organoids from Trpm5GFP:Gpr64mCherry mice revealed sequential expression of initially Trpm5GFP and subsequently also Gpr64mCherry in maturing intestinal tuft cells.
[Molecular Metabolism]
Grunddal, K. V., Tonack, S., Egerod, K. L., Thompson, J. J., Petersen, N., Engelstoft, M. S., Vagne, C., Keime, C., Gradwohl, G., Offermanns, S., & Schwartz, T. W. (2021). Adhesion Receptor Adgrg2/Gpr64 Is In The Gi-Tract Selectively Expressed In Mature Intestinal Tuft Cells. Molecular Metabolism, 101231. https://doi.org/10.1016/j.molmet.2021.101231 Cite
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Improved Radium-223 Therapy with Combination Epithelial Sodium Channel Blockade

Utilizing primary human duodenal organoids as in vitro models of the functional GI epithelium, researchers found that Amiloride and NS-1619 presented significant effects in 223Ra membranal transport.
[Journal of Nuclear Medicine]
Abou, D. S., Fears, A., Summer, L., Longtine, M., Benabdallah, N., Riddle, R. C., Ulmert, D., Michalski, J., Wahl, R. L., Chesner, D., Doucet, M., Zachos, N., Simons, B., & Thorek, D. L. (2021). Improved Radium-223 Therapy with Combination Epithelial Sodium Channel Blockade. Journal of Nuclear Medicine. https://doi.org/10.2967/jnumed.121.261977 Cite
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Therapeutic B-Cell Depletion Reverses Progression of Alzheimerā€™s Disease

Using three different murine transgenic models, scientists provide counterintuitive evidence that the Alzheimerā€™s disease progression required B cells.
[Nature Communications]
Kim, K., Wang, X., Ragonnaud, E., Bodogai, M., Illouz, T., DeLuca, M., McDevitt, R. A., Gusev, F., Okun, E., Rogaev, E., & Biragyn, A. (2021). Therapeutic B-cell depletion reverses progression of Alzheimer’s disease. Nature Communications, 12(1), 2185. https://doi.org/10.1038/s41467-021-22479-4 Cite
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The IRENA lncRNA Converts Chemotherapy-Polarized Tumor-Suppressing Macrophages to Tumor-Promoting Phenotypes in Breast Cancer

Scientists found that IFN-activated proinflammatory macrophages after neoadjuvant chemotherapy enhanced antitumor immunity but promoted cancer chemoresistance.
[Nature Cancer]
Liu, J., Lao, L., Chen, J., Li, J., Zeng, W., Zhu, X., Li, J., Chen, X., Yang, L., Xing, Y., Chen, F., Huang, D., Zhang, X., Wei, W., Gong, C., Huang, S., Yu, Z., Li, Z., Yang, L., … Song, E. (2021). The IRENA lncRNA converts chemotherapy-polarized tumor-suppressing macrophages to tumor-promoting phenotypes in breast cancer. Nature Cancer, 1–17. https://doi.org/10.1038/s43018-021-00196-7 Cite
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Nicotinamide Riboside, an NAD+ Precursor, Attenuates Inflammation and Oxidative Stress by Activating Sirtuin 1 in Alcohol-Stimulated Macrophages

Researchers investigated the effects of nicotinamide riboside, a natural nicotinamide adenine dinucleotide precursor, on alcohol-induced inflammation and oxidative stress in macrophages.
[Laboratory Investigation]
Kang, H., Park, Y.-K., & Lee, J.-Y. (2021). Nicotinamide riboside, an NAD + precursor, attenuates inflammation and oxidative stress by activating sirtuin 1 in alcohol-stimulated macrophages. Laboratory Investigation, 1–13. https://doi.org/10.1038/s41374-021-00599-1 Cite
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Silk Fibroin Enhances Cytocompatibilty and Dimensional Stability of Alginate Hydrogels for Light-Based Three-Dimensional Bioprinting

Alginate derivatives were synthesized to induce rapid gelation, and a bioink was prepared by mixing these alginate derivatives with silk fibroin to enhance cell compatibility.
[Biomacromolecules]
Kim, E., Seok, J. M., Bae, S. B., Park, S. A., & Park, W. H. (2021). Silk Fibroin Enhances Cytocompatibilty and Dimensional Stability of Alginate Hydrogels for Light-Based Three-Dimensional Bioprinting. Biomacromolecules. https://doi.org/10.1021/acs.biomac.1c00034 Cite
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A Liquid Culture Cancer Spheroid Model Reveals Low PI3K/Akt Pathway Activity and Low Adhesiveness to the Extracellular Matrix

Scientists examined liquid culture cancer spheroids for the acquisition of apicalā€basal polarity, sensitivity to an Akt inhibitor and interaction with extracellular matrix.
[FEBS Journal]
Abe‐Fukasawa, N., Watanabe, R., Gen, Y., Nishino, T., & Itasaki, N. (n.d.). A liquid culture cancer spheroid model reveals low PI3K/Akt pathway activity and low adhesiveness to the extracellular matrix. The FEBS Journal, n/a(n/a). https://doi.org/https://doi.org/10.1111/febs.15867 Cite
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