Scientists designed two triplex droplet digital PCR assays, each with two unique targets and one in common, and normalize the results to PCR-based T cell counts.
[Cell Reports Medicine]
Levy, C. N., Hughes, S. M., Roychoudhury, P., Reeves, D. B., Amstuz, C., Zhu, H., Huang, M.-L., Wei, Y., Bull, M. E., Cassidy, N. A. J., McClure, J., Frenkel, L. M., Stone, M., Bakkour, S., Wonderlich, E. R., Busch, M. P., Deeks, S. G., Schiffer, J. T., Coombs, R. W., … Hladik, F. (2021). A highly multiplexed droplet digital PCR assay to measure the intact HIV-1 proviral reservoir. Cell Reports Medicine, 0(0). https://doi.org/10.1016/j.xcrm.2021.100243 Cite
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