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Isolation of Porcine Adult Cardiomyocytes: Comparison between Langendorff Perfusion and Tissue Slicing-Assisted Enzyme Digestion

[PLOS One] Using adult Bama minipigs, scientists performed cardiomyocyte isolation via two distinct methods, and compared the resulting cellular quality, including viability, cellular structure, gene expression, and electrophysiological properties, of cardiomyocytes from three distinct anatomical regions.

Myoglobin-Derived Iron Causes Wound Enlargement and Impaired Regeneration in Pressure Injuries of Muscle

[Elife] Researchers examined the impact of myoglobin (Mb) iron in murine muscle pressure injuries. Tests used Mb-knockout or treatment with deferoxamine iron chelator.

GLP-1 Receptor Agonist Protects Palmitate-Induced Insulin Resistance in Skeletal Muscle Cells by Up-Regulating sestrin2 to Promote Autophagy

[Scientific Reports] Scientists investigated whether liraglutide could effectively reduce insulin resistance (IR) by regulating sestrin2 (SESN2) expression in L6 rat skeletal muscle cells by examining its interactions with SESN2, autophagy, and IR.

Biochemical and Functional Characterization of Skeletal Muscle Cells Differentiated from Tonsil-Derived Mesenchymal Stem Cells

[Muscle & Nerve] Investigators examined whether skeletal muscle cells derived from tonsil mesenchymal stem cells formed a neuromuscular junction when cocultured with motor neurons, and whether they were stimulated by electrical signals using whole-cell patch clamping.

Crosstalk between Diacylglycerol Kinase and Protein Kinase A in the Regulation of Airway Smooth Muscle Cell Proliferation

[Respiratory Research] Scientists assayed cell proliferation using CyQUANTâ„¢ NF assay, protein expression and phosphorylation using immunoblotting, and prostaglandin E2 secretion by ELISA.

Quercetin Attenuates Angiotensin II-Induced Proliferation of Vascular Smooth Muscle Cells and p53 Pathway Activation In Vitro and In Vivo

[Biofactors] In vitro, quercetin treatment meaningfully decreased the cell viability, arrested cell cycle at G0/G1 phase, and up-regulated the p53 and p21 proteins expression, as well as down-regulated the protein expression of cell cycle-related markers in angiotensin II stimulated vascular smooth muscle cells.

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