Endothelial Cells Control Vascular Smooth Muscle Cell Cholesterol Levels by Regulating 24-Dehydrocholesterol Reductase Expression

The authors investigated changes of the vascular smooth muscle cell (VSMC) transcriptome by utilizing 3D human vascular organoids organized as a core of VSMCs enclosed by a monolayer of endothelial cells.
[Experimental Cell Research]
Kohlhaas, J., Jäger, M., Lust, L., De La Torre, C., Hecker, M., & Korff, T. (2021). Endothelial cells control vascular smooth muscle cell cholesterol levels by regulating 24-dehydrocholesterol reductase expression. Experimental Cell Research, 112446. https://doi.org/10.1016/j.yexcr.2020.112446 Cite
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LOX-1 and Cancer: An Indissoluble Liaison

The authors outline the role of LOX-1 in tumor spreading and metastasis, evidencing its function in VEGF induction, HIF-1alpha activation, and MMP-9/MMP-2 expression, pushing up the neoangiogenic and the epithelial-mesenchymal transition process in glioblastoma, osteosarcoma, prostate, colon, breast, lung, and pancreatic tumors.
[Cancer Gene Therapy]
Murdocca, M., De Masi, C., Pucci, S., Mango, R., Novelli, G., Di Natale, C., & Sangiuolo, F. (2021). LOX-1 and cancer: an indissoluble liaison. Cancer Gene Therapy, 1–11. https://doi.org/10.1038/s41417-020-00279-0 Cite
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Dasatinib Induces Endothelial-to-Mesenchymal Transition in Human Vascular-Endothelial Cells: Counteracted by Cotreatment with Bosutinib

The authors investigated the effects of tyrosine kinase inhibitors (TKIs) on the development of endothelial-to-mesenchymal transition in human vascular-endothelial cells (VECs). Exposure of VECs to dasatinib, but not to other TKIs, produced a significant increase in the formation of spindle-shaped cells.
[International Journal of Hematology]
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miR-217 Promotes Endothelial Cell Senescence through the SIRT1/p53 Signaling Pathway

Human umbilical vein endothelial cells (HUVECs) were used to replicate the aging model, and the population doubling levels during cell passage were counted. Senescence-associated β-galactosidase staining, real-time quantitative PCR, MTT assay, Transwell, and tube formation were used to detect the effects of miR-217 on young and senescent HUVECs.
[Journal of Molecular Histology]
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Identification of a Human Whole Blood-Based Endothelial Cell Impedance Assay for Assessing Clinical TRPV4 Target Engagement Ex Vivo

The ability of GSK2798745 to block TRPV4 agonist GSK1016790-induced impendence reduction in human umbilical vein endothelial cells in the presence of human whole blood was assessed.
[Journal of Pharmacology and Experimental Therapeutics]
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Fruquintinib Inhibits VEGF/VEGFR2 Axis of Choroidal Endothelial Cells and M1-Type Macrophages to Protect against Mouse Laser-Induced Choroidal Neovascularization

Investigators HMPL-013 inhibited VEGF/VEGFR2 binding in choroidal endothelial cells and macrophages, as well as macrophage M1 polarization. In vitro, noncontact coculture of human choroidal vascular endothelial cells and macrophages under hypoxia conditions was established.
[Cell Death & Disease]
Liu, X., Guo, A., Tu, Y., Li, W., Li, L., Liu, W., Ju, Y., Zhou, Y., Sang, A., & Zhu, M. (2020). Fruquintinib inhibits VEGF/VEGFR2 axis of choroidal endothelial cells and M1-type macrophages to protect against mouse laser-induced choroidal neovascularization. Cell Death & Disease, 11(11), 1–13. https://doi.org/10.1038/s41419-020-03222-1 Cite
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Immunobiology and Immunotherapy of HCC: Spotlight on Endothelial Specific YY1 Deletion Restricts Tumor Angiogenesis and Tumor Growthinnate and Innate-Like Immune Cells

VEGF-induced endothelial cell (EC) migration was diminished in Yin Yang 1 (YY1)-depleted HUVECs. A rescue experiment revealed that YY1-regulated BMP6 expression in ECs was involved in EC migration.
[Scientific Reports]
Ruf, B., Heinrich, B., & Greten, T. F. (2020). Immunobiology and immunotherapy of HCC: spotlight on innate and innate-like immune cells. Cellular & Molecular Immunology, 1–16. https://doi.org/10.1038/s41423-020-00572-w Cite
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RNA Sequencing Reveals BMP4 as a Basis for the Dual-Target Treatment of Diabetic Retinopathy

Researchers explored the pathogenesis of diabetic retinopathand identified new treatments that can provide dual-target intervention for angiogenesis and fibrosis. They explored changes in gene expression in high glucose–induced vascular endothelial cells using RNA sequencing technology.
[International Journal of Molecular Medicine]
Dong, L., Zhang, Z., Liu, X., Wang, Q., Hong, Y., Li, X., & Liu, J. (2020). RNA sequencing reveals BMP4 as a basis for the dual-target treatment of diabetic retinopathy. Journal of Molecular Medicine. https://doi.org/10.1007/s00109-020-01995-8 Cite
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miRNA-126-3p Carried by Human Umbilical Cord Mesenchymal Stem Cell Enhances Endothelial Function through Exosome-Mediated Mechanisms In Vitro and Attenuates Vein Graft Neointimal Formation In Vivo

Human umbilical cord MSCs (hucMSCs) and human umbilical vein endothelial cells (HUVECs) were isolated from human umbilical cords and characterized by a series of experiments. Lentivirus vector encoding miRNA-126-3p was transfected into hucMSCs and verified by PCR.
[Stem Cell Research & Therapy]
Qu, Q., Wang, L., Bing, W., Bi, Y., Zhang, C., Jing, X., & Liu, L. (2020). miRNA-126-3p carried by human umbilical cord mesenchymal stem cell enhances endothelial function through exosome-mediated mechanisms in vitro and attenuates vein graft neointimal formation in vivo. Stem Cell Research & Therapy, 11(1), 464. https://doi.org/10.1186/s13287-020-01978-z Cite
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Pyruvate Kinase M2 Mediates the Glycolysis in the Lymphatic Endothelial Cells and Its Potential Relation to the Progression of Lymphatic Malformations

The glycolysis index, including glucose uptake, ATP and lactate production stayed at a relatively high level in human dermal lymphatic endothelial cells (HDLECs) compared with HUVECS, while inhibition of PKM2 by shikinon or PKM2 knockdown could significantly suppress glycolysis, migration, tubular formation, and invasion of HDLECs.
[American Journal of Pathology]
Jiang, H., Zou, Y., Zhao, J., Li, X., Yang, S., Zhou, X., Mou, D., Zhong, W., & Cai, Y. (2020). Pyruvate kinase M2 mediates the glycolysis in the lymphatic endothelial cells and its potential relation to the progression of lymphatic malformations. The American Journal of Pathology, 0(0). https://doi.org/10.1016/j.ajpath.2020.10.003 Cite
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Fibroblast Growth Factor 2 Accelerates the Epithelial–Mesenchymal Transition in Keratinocytes During Wound Healing Process

Fibroblast growth factor (FGF) 2, also called basic FGF, had the potential to accelerate wound closure by activating vascular endothelial cells and fibroblasts. Scientists examined the effects of FGF2 on keratinocyte morphology and epithelial–mesenchymal transition in wounded skin.
[Scientific Reports]
Koike, Y., Yozaki, M., Utani, A., & Murota, H. (2020). Fibroblast growth factor 2 accelerates the epithelial–mesenchymal transition in keratinocytes during wound healing process. Scientific Reports, 10(1), 18545. https://doi.org/10.1038/s41598-020-75584-7 Cite
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