western blot analysis

[Molecular Therapy-Nucleic Acids] RNA sequencing, qRT-PCR, and Western blot analysis were used to identify the aberrant expression and signaling of miR-148a-3p within cells, mice, and patients with Alzheimer’s disease (AD). Researchers implicated the miR-148a-3p/p35/PTEN pathway as an essential contributor to tau hyperphosphorylation and feed-forward regulation in AD.

[Aging] The authors investigated the effects of bone morphogenetic protein 9 on osteogenesis and angiogenesis of human amniotic mesenchymal stem cells (hAMSCs) cocultured with human umbilical vein endothelial cells (HUVECs) and determined the possible underlying molecular mechanism.

[Journal of Cellular Biochemistry] Scientists revealed the differences in methionine metabolism of bone marrow (BM)-mesenchymal stem cells (MSCs), umbilical cord blood-MSCs, and cancer stem cells, which reflect different metabolic profiles and developmental stages of stem cells.

[Diabetes] Researchers reported increased expression of miR-200c in islets from type 2 diabetes as compared with non-diabetic donors and displayed results showing reduced glucose-stimulated insulin secretion in EndoC-βH1 cells overexpressing miR-200c.

[Cell Death & Disease] By exposing bEnd.3 endothelial cells and primary cortical neurons to oxygen−glucose deprivation (OGD), the impact of extracellular vesicles from OGD-preconditioned microglia on angiogenesis and neuronal apoptosis by the tube formation assay and TUNEL staining was assessed.

[Cell Biology International] Scientists investigated the expression of N-Cadherin, a trans-membrane protein that enhanced the cell adhesion and recently gained attention for differentiation and augmentation of stem cell function, in mouse cardiac MSCs.