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western blot analysis

MiR-571 Affects the Development and Progression of Liver Fibrosis by Regulating the Notch3 Pathway

[Scientific Reports] Investigators found that miR-571 inhibited the apoptosis of human hepatic stellate cells and promoted cell proliferation and migration. Its up regulation promoted the expression of Notch3 and Jagged1, and promoted the expression of related fibroblasts.

CD146 Mediates the Anti-apoptotic Role of Netrin-1 in Endothelial Progenitor Cells under Hypoxic Conditions

[Molecular Medicine Reports] Researchers explored the role and molecular mechanisms underlying Netrin‑1 in the viability and angiogenic function of EPCs. The apoptosis and various functions of EPCs were analyzed in vitro by manipulating the expression of Netrin‑1.

Negative Inotropic Mechanisms of β-Cardiotoxin in Cardiomyocytes by Depression of Myofilament ATPase Activity without Activation of the Classical β-Adrenergic Pathway

[Scientific Reports] The effects of beta-cardiotoxin on isolated cardiac myocyte functions, calcium homeostasis, the phosphorylation level of targeted proteins, and the myofibrillar ATPase activity were studied.

Dysregulation of the miR-30a/BiP Axis by Cigarette Smoking Accelerates Oral Cancer Progression

[Cancer Cell International] The effects of conditioned media from oral squamous cell carcinoma cells on the angiogenic activities of endothelial cells were analyzed using a tube formation assay.

DLX6-AS1 Activated by H3K4me1 Enhanced Secondary Cisplatin Resistance of Lung Squamous Cell Carcinoma through Modulating miR-181a-5p/miR-382-5p/CELF1 Axis

[Scientific Reports] Secondary cisplatin resistance majorly undermines the cisplatin efficacy leading to a worse prognosis. In this respect, researchers identified the role of the DLX6-AS1/miR-181a-5p/miR-382-5p/CELF1 axis in regulating cisplatin resistance of lung squamous cell carcinoma.

Podophyllotoxin Induces ROS-Mediated Apoptosis and Cell Cycle Arrest in Human Colorectal Cancer Cells via p38 MAPK Signaling

[Biomolecules & Therapeutics] Researchers evaluated the anticancer effect of Podophyllotoxin on human colorectal cancer HCT116 cells and identified the detailed molecular mechanism.

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