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western blot analysis

Hypoxia Increases KIAA1199/CEMIP Expression and Enhances Cell Migration in Pancreatic Cancer

[Scientific Reports] Using quantitative real-time RT-PCR and western blot analysis, scientists analysed the changes in the expression of HA-synthesizing enzymesand hyaluronan HA-degrading enzymes in pancreatic ductal adenocarcinoma cell lines under hypoxic conditions.

Pancreatic Cancer Cell-Derived MicroRNA-155-5p-Containing Extracellular Vesicles Promote Immune Evasion by Triggering EHF-Dependent Activation of Akt/NF-κB Signaling Pathway

[International Immunopharmacology] Ectopic expression and knockdown experiments were conducted in pancreatic cancer (PC) cells, PC cells-derived EVs, or mouse xenograft model of PC.

Serum-Derived Exosomes from House Dust Mite-Sensitized Guinea Pigs Contribute to Inflammation in BEAS-2B Cells via the TLR4-NF-κB Pathway

[Molecular Medicine Reports] The authors investigated whether serum exosomes played a pro‑inflammatory role in bronchial epithelial cells and explored the underlying molecular mechanisms.

Melatonin Restores Osteoporosis-Impaired Osteogenic Potential of Bone Marrow Mesenchymal Stem Cells and Alleviates Bone Loss through the HGF/PTEN/Wnt/β-Catenin Axis

[Therapeutic Advances in Chronic Disease] Real-time qualitative PCR and Western blot analysis were conducted to determine expression of HGF, PTEN, and osteoblast differentiation-related genes in ovariectomy-induced osteoporosis mice and the isolated bone marrow MSCs.

The Relationship between Melanin Production and Lipofuscin Formation in Tyrosinase Gene Knockout Melanocytes Using CRISPR/Cas9 System

[Life Sciences] Scientists investigated the relationship between melanin production and lipofuscin synthesis in normal mouse melanoma cell line B16F1 cells and Tyrosinase gene knockout cells.

iTRAQ-Based Proteome Analysis of Porcine Group a Rotavirus-Infected Porcine IPEC-J2 Intestinal Epithelial Cells

[Journal of Proteomics] The authors investigated the protein profile of porcine rotavirus (PoRV)-infected IPEC-J2 cells using quantitative proteomics and provided valuable information to better understand the mechanisms underlying the host responses to PoRV infection in piglets.

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