TY - JOUR TI - Mesenchymal stem cells-derived and siRNAs-encapsulated exosomes inhibit osteonecrosis of the femoral head AU - Zhang, Chi AU - Su, Yan AU - Ding, Hao AU - Yin, Jimin AU - Zhu, Zhenhong AU - Song, Wenqi T2 - Journal of Cellular and Molecular Medicine AB - Osteonecrosis of the femoral head (ONFH) is a progressive, obstinate and disabling disease. At present, the treatment of ONFH is still a global medical problem. We aim to explore the role of bone mesenchymal stem cells (BMSCs)-derived and siRNAs-encapsulated exosomes (siRNAs-encapsulated BMSCexos) in ONFH. We first isolated BMSCexos and screened siRNAs of 6 ONFH-related genes for siRNAs-encapsulated BMSCexo. The expression of these 6 ONFH-related genes in dexamethasone (DXM)-treated MC3T3-E1 cell, cell model of ONFH, was detected by RT-qPCR and Western blot analysis. And then, we performed CCK-8 assay, angiogenesis assay and HE staining analysis to test the promotion role of the siRNAs-encapsulated BMSCexo for angiogenesis during ONFH repair. The results suggest that the obtained particles were BMSCexos. The screened effective siRNAs could effectively knock down their expression in VECs. Moreover, siRNAs-encapsulated BMSCexo could effectively knock down the expression of these genes in VECs. In addition, siRNAs-encapsulated BMSCexo promote angiogenesis during ONFH repair. In conclusion, we found siRNAs-encapsulated BMSCexos could promote ONFH repair by angiogenesis, and indicated exosome as the new siRNA carrier is of great significance to improve the efficiency of RNAi. DO - 10.1111/jcmm.15395 DP - Wiley Online Library VL - n/a IS - n/a LA - en SN - 1582-4934 UR - https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.15395 Y2 - 2020/08/06/17:46:32 KW - BMSCs KW - ONFH KW - siRNAs-encapsulated BMSCexos ER -