TY - JOUR TI - Bone marrow mesenchymal stem cells-derived exosomal microRNA-193a reduces cisplatin resistance of non-small cell lung cancer cells via targeting LRRC1 AU - Wu, Hongbo AU - Mu, Xiaoqian AU - Liu, Lei AU - Wu, Huijuan AU - Hu, Xiufeng AU - Chen, Lijuan AU - Liu, Jie AU - Mu, Yu AU - Yuan, Fangfang AU - Liu, Wenjing AU - Zhao, Yanqiu T2 - Cell Death & Disease AB - Exosomes are small endogenous membrane vesicles that can mediate cell communication by transferring genetic materials. Based on that, exosomes have always been discussed as a cargo carrier for microRNA (miRNA) transportation. Accumulating data have reported the inhibitory effects of microRNA-193a (miR-193a) on non-small cell lung cancer (NSCLC) cell progression. However, the mechanisms of miR-193a delivery to cancer cells and miR-193a in exosomes have not been explored clearly in NSCLC. Given that, this work aims to decode exosomal miR-193a in cisplatin (DDP) resistance of NSCLC cells. A549 and H1299 cell lines were screened out and their parent cells and drug-resistant cells were co-cultured with human bone marrow mesenchymal stem cells (BMSCs)-derived exosomes (BMSC-Exo) that had been transfected with miR-193a mimic or si-LRRC1 to detect the colony formation, migration, apoptosis, invasion and proliferation of NSCLC cells. In vivo experiment was conducted to verify the in vitro results. BMSC-Exo with upregulated miR-193a and downregulated LRRC1 suppressed colony formation, invasion, proliferation and migration as well as advanced apoptosis of NSCLC parent cells and drug-resistant cells. BMSC-Exo combined with upregulated miR-193a reduced tumor volume and weight in mice with NSCLC. Functional studies report that BMSC-Exo shuffle miR-193a to suppress the colony formation, invasion, migration, and proliferation as well as advance apoptosis of NSCLC DDP-resistant cells via downregulating LRRC1. DA - 2020/09/25/ PY - 2020 DO - 10.1038/s41419-020-02962-4 DP - www.nature.com VL - 11 IS - 9 SP - 1 EP - 14 LA - en SN - 2041-4889 UR - https://www.nature.com/articles/s41419-020-02962-4 Y2 - 2020/09/25/17:16:15 ER -