TY - JOUR TI - Comparative stemness and differentiation of luminal and basal breast cancer stem cell type under glutamine‐deprivation AU - Jariyal, Heena AU - Gupta, Chanchal AU - Andhale, Shambhavi AU - Gadge, Sonali AU - Srivastava, Akshay T2 - Journal of Cell Communication and Signaling AB - Glutamine (gln) metabolism has emerged as a cancer therapeutic target in past few years, however, the effect of gln-deprivation of bCSCs remains elusive in breast cancer. In this study, effect of glutamine on stemness and differentiation potential of bCSCs isolated from MCF-7 and MDAMB-231 were studied. We have shown that bCSCs differentiate into CD24+ epithelial population under gln-deprivation and demonstrated increased expression of epithelial markers such as e-cadherin, claudin-1 and decreased expression of mesenchymal protein n-cadherin. MCF-7-bCSCs showed a decrease in EpCAMhigh population whereas MDAMB-231-bCSCs increased CD44high population in response to gln-deprivation. The expression of intracellular stem cell markers such sox-2, oct-4 and nanog showed a drastic decrease in gene expression under gln-deprived MDAMB-231-bCSCs. Finally, localization of β-catenin in MCF-7 and MDAMB-231 cells showed its accumulation in cytosol or perinuclear space reducing its efficiency to transcribe downstream genes. Conclusively, our study demonstrated that gln-deprivation induces differentiation of bCSCs into epithelial subtypes and also reduces stemness of bCSCs mediated by reduced nuclear localization of β-catenin. It also suggests that basal and luminal bCSCs respond differentially towards changes in extracellular and intracellular gln. This study could significantly affect the gln targeting regimen of breast cancer therapeutics. DA - 2021/01/28/ PY - 2021 DO - 10.1007/s12079-020-00603-1 DP - Springer Link J2 - J. Cell Commun. Signal. LA - en SN - 1873-961X UR - https://doi.org/10.1007/s12079-020-00603-1 Y2 - 2021/02/01/21:10:35 ER -