TY - JOUR TI - Therapeutic effects of Wharton’s jelly-derived Mesenchymal Stromal Cells on behaviors, EEG changes and NGF-1 in rat model of the Parkinson’s disease AU - Jalali, Maryam Sadat AU - Saki, Ghasem AU - Farbood, Yaghoub AU - Azandeh, Seyed saeed AU - Mansouri, Esrafil AU - Ghasemi Dehcheshmeh, Mohammad AU - Sarkaki, Alireza T2 - Journal of Chemical Neuroanatomy AB - Human Wharton's jelly-derived Mesenchymal Stromal Cells (hWJ-MSCs) have shown beneficial effects in improving the dopaminergic cells in the Parkinson’s disease (PD). In the present study, the effects of hWJ-MSCs on hyperalgesia, anxiety deficiency and Pallidal local electroencephalogram (EEG) impairment, alone and combined with L-dopa, were examined in a rat model of PD. Adult male Wistar rats were divided into five groups: 1) sham, 2) PD, 3) PD + C (Cell therapy), 4) PD + C+D (Drug), and 5) PD + D. PD was induced by injection of 6-OHDA (16 μg/2 μl into medial forebrain bundle (MFB)). PD + C group received hWJ-MSCs (1 × 106 cells, intravenous (i.v.)) twice post PD induction. PD + C+D groups received hWJ-MSCs combined with L-Dopa/Carbidopa, (10/30 mg/kg, intraperitoneally (i.p.)). PD + D group received L-Dopa/Carbidopa alone. Four months later, analgesia, anxiety-like behaviors, were evaluated and Pallidal local EEG was recorded. Level of insulin-like growth factor 1 (IGF-1) was measured in the striatum and dopaminergic neurons were counted in substantia nigra (SNc). According to data, MFB-lesioned rats showed hyperalgesia in tail flick, anxiety-like symptoms in cognitive tests, impairment of electrical power of pallidal local EEG as field potential, count of dopaminergic neurons in SNc and level of IGF-1 in striatum. These complications restored significantly by MSCs treatment (p < 0.001). Our findings confirm that chronic treatment with hWJ-MSC, alone and in combination with L-Dopa, improved nociception and cognitive deficit in PD rats which may be the result of increasing IGF-1 and protect the viability of dopaminergic neurons. DA - 2021/04/01/ PY - 2021 DO - 10.1016/j.jchemneu.2021.101921 DP - ScienceDirect VL - 113 SP - 101921 J2 - Journal of Chemical Neuroanatomy LA - en SN - 0891-0618 UR - https://www.sciencedirect.com/science/article/pii/S0891061821000041 Y2 - 2021/02/23/18:59:46 KW - Anxiety KW - IGF-1 KW - Local EEG KW - Parkinson’s disease KW - Stem cells ER -