TY - JOUR TI - In vitro co-culture of human intestinal organoids and lamina propria-derived CD4+ T cells AU - Schreurs, Renée R. C. E. AU - Baumdick, Martin E. AU - Drewniak, Agata AU - Bunders, Madeleine J. T2 - STAR Protocols AB - Crosstalk between immune cells and intestinal stem cells (ISCs) in vivo plays a critical role in tissue homeostasis and inflammation; however, in vitro models based on primary cells recapitulating this interaction were lacking. Here, we provide a detailed protocol for an autologous in vitro long-term 3D co-culture system of human intestinal CD4+ T cells and ISCs to study T cell-intestinal epithelial cell interactions during tissue development and inflammation. For complete details on the use and execution of this protocol, please refer to Schreurs et al. (2019). DA - 2021/06/18/ PY - 2021 DO - 10.1016/j.xpro.2021.100519 DP - ScienceDirect VL - 2 IS - 2 SP - 100519 J2 - STAR Protocols LA - en SN - 2666-1667 UR - https://www.sciencedirect.com/science/article/pii/S2666166721002264 Y2 - 2021/05/27/21:56:09 KW - Cell culture KW - Immunology KW - Organoids ER -