TY - JOUR TI - Galectin-3 Expression in Pancreatic Cell Lines Under Distinct Autophagy-Inducing Stimulus AU - Filho, Antônio Felix da Silva AU - Sousa, Lizandra Maia de AU - Consonni, Silvio Roberto AU - Pitta, Maira Galdino da Rocha AU - Carvalho, Hernandes Faustino AU - Rêgo, Moacyr Jesus Barreto de Melo T2 - Microscopy and Microanalysis AB - , Hypoxia and nutrient deprivation are responsible for inducing malignant behavior in neoplastic cells. In these conditions, metabolic stress leads the cells to enhance their autophagic flux and to activate key molecules for homeostasis maintenance. Galectin-3 (Gal-3) is upregulated in pancreatic cancer and it is activated under the hypoxic atmosphere. We aimed to analyze the most effective autophagic-inducing conditions in pancreatic ductal adenocarcinoma cells and the effect exerted under these conditions in association with hypoxia on the Gal-3 expression. Gal-3 and the microtubule-associated protein light chain 3 beta (LC3) were accessed through western blot and immunofluorescence. Degradative vacuole quantification was analyzed by transmission electronic microscopy, and inhibition of Gal-3 was performed using siRNA. According to the analyses, the most effective conditions in the inducement of autophagy for PANC-1 and MIA PaCa-2 cells were nutritional deprivation and complete amino acid/glucose deprivation, respectively. PANC-1 cells presented higher Gal-3 when they were submitted to 24 h of nutritional deprivation alone and simultaneously nutritional and oxygen deprivation. Inhibition of Gal-3 causes a decrease of LC3 levels in all experimental conditions. These results confirm that Gal-3 is modulated by microenvironment factors and the possibility of Gal-3 participating in an adaptive response from PDAC cells to extreme conditions. DA - undefined/ed PY - undefined/ed DO - 10.1017/S1431927620024526 DP - Cambridge University Press SP - 1 EP - 11 LA - en SN - 1431-9276, 1435-8115 UR - https://www.cambridge.org/core/journals/microscopy-and-microanalysis/article/galectin3-expression-in-pancreatic-cell-lines-under-distinct-autophagyinducing-stimulus/C0F7FD34DB3E6DECB3479BB7EC603636 Y2 - 2020/10/27/17:01:46 KW - MIA PaCa-2 KW - PANC-1 KW - hypoxia KW - microenvironment KW - pancreatic ductal adenocarcinoma ER -