TY - JOUR TI - Antigen retrieval and clearing for whole-organ immunofluorescence by FLASH AU - Messal, Hendrik A. AU - Almagro, Jorge AU - Zaw Thin, May AU - Tedeschi, Antonio AU - Ciccarelli, Alessandro AU - Blackie, Laura AU - Anderson, Kurt I. AU - Miguel-Aliaga, Irene AU - van Rheenen, Jacco AU - Behrens, Axel T2 - Nature Protocols AB - Advances in light-sheet and confocal microscopy now allow imaging of cleared large biological tissue samples and enable the 3D appreciation of cell and protein localization in their native organ environment. However, the sample preparations for such imaging are often onerous, and their capability for antigen detection is limited. Here, we describe FLASH (fast light-microscopic analysis of antibody-stained whole organs), a simple, rapid, fully customizable technique for molecular phenotyping of intact tissue volumes. FLASH utilizes non-degradative epitope recovery and membrane solubilization to enable the detection of a multitude of membranous, cytoplasmic and nuclear antigens in whole mouse organs and embryos, human biopsies, organoids and Drosophila. Retrieval and immunolabeling of epithelial markers, an obstacle for previous clearing techniques, can be achieved with FLASH. Upon volumetric imaging, FLASH-processed samples preserve their architecture and integrity and can be paraffin-embedded for subsequent histopathological analysis. The technique can be performed by scientists trained in light microscopy and yields results in <1 week. DA - 2020/11/27/ PY - 2020 DO - 10.1038/s41596-020-00414-z DP - www.nature.com SP - 1 EP - 33 LA - en SN - 1750-2799 UR - https://www.nature.com/articles/s41596-020-00414-z Y2 - 2020/12/01/00:45:56 ER -