TY - JOUR TI - Canagliflozin attenuates isoprenaline-induced cardiac oxidative stress by stimulating multiple antioxidant and anti-inflammatory signaling pathways AU - Hasan, Raquibul AU - Lasker, Shoumen AU - Hasan, Ahasanul AU - Zerin, Farzana AU - Zamila, Mushfera AU - Chowdhury, Faizul Islam AU - Nayan, Shariful Islam AU - Rahman, Md Mizanur AU - Khan, Ferdous AU - Subhan, Nusrat AU - Alam, Md Ashraful T2 - Scientific Reports AB - The antidiabetic drug canagliflozin is reported to possess several cardioprotective effects. However, no studies have investigated protective effects of canagliflozin in isoprenaline (ISO)-induced cardiac oxidative damage—a model mimicking sympathetic nervous system (SNS) overstimulation-evoked cardiac injuries in humans. Therefore, we investigated protective effects of canagliflozin in ISO-induced cardiac oxidative stress, and their underlying molecular mechanisms in Long-Evans rat heart and in HL-1 cardiomyocyte cell line. Our data showed that ISO administration inflicts pro-oxidative changes in heart by stimulating production of reactive oxygen species (ROS) and reactive nitrogen species (RNS). In contrast, canagliflozin treatment in ISO rats not only preserves endogenous antioxidants but also reduces cardiac oxidative stress markers, fibrosis and apoptosis. Our Western blotting and messenger RNA expression data demonstrated that canagliflozin augments antioxidant and anti-inflammatory signaling involving AMP-activated protein kinase (AMPK), Akt, endothelial nitric oxide synthase (eNOS), nuclear factor erythroid 2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1). In addition, canagliflozin treatment attenuates pro-oxidative, pro-inflammatory and pro-apoptotic signaling mediated by inducible nitric oxide synthase (iNOS), transforming growth factor beta (TGF-β), NADPH oxidase isoform 4 (Nox4), caspase-3 and Bax. Consistently, canagliflozin treatment improves heart function marker in ISO-treated rats. In summary, we demonstrated that canagliflozin produces cardioprotective actions by promoting multiple antioxidant and anti-inflammatory signaling. DA - 2020/09/02/ PY - 2020 DO - 10.1038/s41598-020-71449-1 DP - www.nature.com VL - 10 IS - 1 SP - 14459 LA - en SN - 2045-2322 UR - https://www.nature.com/articles/s41598-020-71449-1 Y2 - 2020/09/02/22:08:46 ER -