TY - JOUR TI - A well plate–based multiplexed platform for incorporation of organoids into an organ-on-a-chip system with a perfusable vasculature AU - Lai, Benjamin Fook Lun AU - Lu, Rick Xing Ze AU - Davenport Huyer, Locke AU - Kakinoki, Sachiro AU - Yazbeck, Joshua AU - Wang, Erika Yan AU - Wu, Qinghua AU - Zhang, Boyang AU - Radisic, Milica T2 - Nature Protocols AB - Owing to their high spatiotemporal precision and adaptability to different host cells, organ-on-a-chip systems are showing great promise in drug discovery, developmental biology studies and disease modeling. However, many current micro-engineered biomimetic systems are limited in technological application because of culture media mixing that does not allow direct incorporation of techniques from stem cell biology, such as organoids. Here, we describe a detailed alternative method to cultivate millimeter-scale functional vascularized tissues on a biofabricated platform, termed ‘integrated vasculature for assessing dynamic events’, that enables facile incorporation of organoid technology. Utilizing the 3D stamping technique with a synthetic polymeric elastomer, a scaffold termed ‘AngioTube’ is generated with a central microchannel that has the mechanical stability to support a perfusable vascular system and the self-assembly of various parenchymal tissues. We demonstrate an increase in user familiarity and content analysis by situating the scaffold on a footprint of a 96-well plate. Uniquely, the platform can be used for facile connection of two or more tissue compartments in series through a common vasculature. Built-in micropores enable the studies of cell invasion involved in both angiogenesis and metastasis. We describe how this protocol can be applied to create both vascularized cardiac and hepatic tissues, metastatic breast cancer tissue and personalized pancreatic cancer tissue through incorporation of patient-derived organoids. Platform assembly to populating the scaffold with cells of interest into perfusable functional vascularized tissue will require 12–14 d and an additional 4 d if pre-polymer and master molds are needed. DA - 2021/03/31/ PY - 2021 DO - 10.1038/s41596-020-00490-1 DP - www.nature.com SP - 1 EP - 32 LA - en SN - 1750-2799 UR - https://www.nature.com/articles/s41596-020-00490-1 Y2 - 2021/03/31/19:05:59 ER -