TY - JOUR TI - Axonal Extensions along Corticospinal Tracts from Transplanted Human Cerebral Organoids AU - Kitahara, Takahiro AU - Sakaguchi, Hideya AU - Morizane, Asuka AU - Kikuchi, Tetsuhiro AU - Miyamoto, Susumu AU - Takahashi, Jun T2 - Stem Cell Reports AB - The reconstruction of lost neural circuits by cell replacement is a possible treatment for neurological deficits after cerebral cortex injury. Cerebral organoids can be a novel source for cell transplantation, but because the cellular composition of the organoids changes along the time course of the development, it remains unclear which developmental stage of the organoids is most suitable for reconstructing the corticospinal tract. Here, we transplanted human embryonic stem cell-derived cerebral organoids at 6 or 10 weeks after differentiation (6w- or 10w-organoids) into mouse cerebral cortices. 6w-organoids extended more axons along the corticospinal tract but caused graft overgrowth with a higher percentage of proliferative cells. Axonal extensions from 10w-organoids were smaller in number but were enhanced when the organoids were grafted 1 week after brain injury. Finally, 10w-organoids extended axons in cynomolgus monkey brains. These results contribute to the development of a cell-replacement therapy for brain injury and stroke. DA - 2020/07/16/ PY - 2020 DO - 10.1016/j.stemcr.2020.06.016 DP - ScienceDirect J2 - Stem Cell Reports LA - en SN - 2213-6711 UR - http://www.sciencedirect.com/science/article/pii/S221367112030237X Y2 - 2020/07/22/17:57:57 KW - axonal extension KW - cell transplantation KW - cerebral cortex KW - cerebral organoid KW - corticospinal tract KW - developmental stage KW - graft overgrowth KW - human pluripotent stem cell KW - nonhuman primate KW - subcerebral projection neuron ER -