TY - JOUR TI - An endometrial organoid model of Chlamydia-epithelial and immune cell interactions AU - Dolat, Lee AU - Valdivia, Raphael H. T2 - Journal of Cell Science AB - Skip to Next Section Our understanding of how the obligate intracellular bacterial pathogen Chlamydia trachomatis reprograms the function of infected cells in the upper genital tract is largely based on observations made in cell culture with transformed epithelial cell lines. Here we describe a primary organoid system derived from endometrial tissue to recapitulate epithelial cell diversity, polarity, and ensuing responses to Chlamydia infection. Using high-resolution and time-lapse microscopy, we catalogue the infection process in organoids from invasion to egress, including the reorganization of the cytoskeleton and positioning of intracellular organelles. We show this model is amenable to screening C. trachomatis mutants for defects in the fusion of pathogenic vacuoles, the recruitment of intracellular organelles, and inhibition of cell death. Moreover, we reconstructed a primary immune cell response by co-culturing infected organoids with neutrophils, and determined that effectors like CPAF and TepP limit the recruitment of neutrophils to infected organoids. Collectively, our model can be applied to study the cell biology of Chlamydia infections in three dimensional structures that better reflect the diversity of cell types and polarity encountered by Chlamydia in their animal hosts. DA - 2021/01/01/ PY - 2021 DO - 10.1242/jcs.252403 DP - jcs.biologists.org J2 - J Cell Sci LA - en SN - 0021-9533, 1477-9137 UR - https://jcs.biologists.org/content/early/2021/01/19/jcs.252403 Y2 - 2021/01/23/01:32:11 ER -