TY - JOUR TI - Establishment of intestinal organoid cultures modeling injury-associated epithelial regeneration AU - Qu, Molong AU - Xiong, Liang AU - Lyu, Yulin AU - Zhang, Xiannian AU - Shen, Jie AU - Guan, Jingyang AU - Chai, Peiyuan AU - Lin, Zhongqing AU - Nie, Boyao AU - Li, Cheng AU - Xu, Jun AU - Deng, Hongkui T2 - Cell Research AB - The capacity of 3D organoids to mimic physiological tissue organization and functionality has provided an invaluable tool to model development and disease in vitro. However, conventional organoid cultures primarily represent the homeostasis of self-organizing stem cells and their derivatives. Here, we established a novel intestinal organoid culture system composed of 8 components, mainly including VPA, EPZ6438, LDN193189, and R-Spondin 1 conditioned medium, which mimics the gut epithelium regeneration that produces hyperplastic crypts following injury; therefore, these organoids were designated hyperplastic intestinal organoids (Hyper-organoids). Single-cell RNA sequencing identified different regenerative stem cell populations in our Hyper-organoids that shared molecular features with in vivo injury-responsive Lgr5+ stem cells or Clu+ revival stem cells. Further analysis revealed that VPA and EPZ6438 were indispensable for epigenome reprogramming and regeneration in Hyper-organoids, which functioned through epigenetically regulating YAP signaling. Furthermore, VPA and EPZ6438 synergistically promoted regenerative response in gut upon damage in vivo. In summary, our results demonstrated a new in vitro organoid model to study epithelial regeneration, highlighting the importance of epigenetic reprogramming that pioneers tissue repair. DA - 2021/01/08/ PY - 2021 DO - 10.1038/s41422-020-00453-x DP - www.nature.com SP - 1 EP - 13 LA - en SN - 1748-7838 UR - https://www.nature.com/articles/s41422-020-00453-x Y2 - 2021/01/08/20:08:30 ER -