TY - JOUR TI - Generation of PARP1 gene knockout human embryonic stem cell line using CRISPR/Cas9 AU - Li, Yuhang AU - Li, Jinbing AU - Zhou, Tiancheng AU - Pan, Guangjin AU - Huang, Ke T2 - Stem Cell Research AB - PARP1 encodes a chromatin-associated enzyme which responsible for post-translational poly(ADP-ribosyl)ation modification (Hsieh et al., 2017). It plays an important role in nucleotide excision repair, non-homologous end joining, DNA mismatch repair and many other DNA repair process. Also, PARP1 participates in inflammation and aging. However, its role in human embryonic stem cell biology has not been fully resolved. To clarify the function of PARP1 in human embryonic stem cells, we reported a PARP1 knockout human embryonic stem cell line, generated by CRISPR/Cas9 mediated gene targeting. This cell line shows normal karyotype, pluripotent stem cell marker expression and differentiation potential in vitro. DA - 2021/05/01/ PY - 2021 DO - 10.1016/j.scr.2021.102288 DP - ScienceDirect VL - 53 SP - 102288 J2 - Stem Cell Research LA - en SN - 1873-5061 UR - https://www.sciencedirect.com/science/article/pii/S1873506121001343 Y2 - 2021/03/24/20:47:42 ER -