TY - JOUR TI - Premalignant oligodendrocyte precursor cells stall in a heterogeneous state of replication stress prior to gliomagenesis AU - Sutcliffe, Matthew D. AU - Galvao, Rui P. AU - Wang, Lixin AU - Kim, Jungeun AU - Rosenfeld, Lauren K. AU - Singh, Shambhavi AU - Zong, Hui AU - Janes, Kevin A. T2 - Cancer Research AB - Cancer evolves from premalignant clones that accumulate mutations and adopt unusual cell states to achieve transformation. We previously pinpointed the oligodendrocyte precursor cell (OPC) as a cell-of-origin for glioma, but the early changes of mutant OPCs during premalignancy remained unknown. Using mice engineered for inducible Nf1-Trp53 loss in OPCs, we acutely isolated labeled mutant OPCs by laser-capture microdissection and determined gene expression changes by bulk RNA sequencing and a fluctuation analysis called stochastic profiling, which uses RNA-sequencing measurements from random pools of 10 mutant cells. At 12 days after Nf1-Trp53 deletion, bulk differences were mostly limited to mitotic hallmarks and genes for ribosome biosynthesis, and stochastic profiling revealed a spectrum of stem-progenitor (Axl, Aldh1a1), proneural, and mesenchymal states as potential starting points for gliomagenesis. At 90 days, bulk sequencing detected few differentially expressed transcripts, whereas stochastic profiling revealed cell states for neurons and mural cells that do not give rise to glial tumors, suggesting cellular dead-ends for gliomagenesis. Importantly, mutant OPCs that strongly expressed key effectors of nonsense-mediated decay (Upf3b) and homology-dependent DNA repair (Rad51c, Slx1b, Ercc4) were identified along with DNA-damage markers, suggesting transcription-associated replication stress. Analysis of 10-cell transcriptomes at 90 days identified a locus of elevated gene expression containing an additional repair endonuclease (Mus81) and Rin1, a Ras-Raf antagonist and possible counterbalance to Nf1 loss. At 150 days, Rin1 was microdeleted in some gliomas and downregulated in all others. In summary, replication stress may pose a considerable bottleneck that must be resolved for glioma initiation. DA - 2021/01/01/ PY - 2021 DO - 10.1158/0008-5472.CAN-20-1037 DP - cancerres.aacrjournals.org J2 - Cancer Res LA - en SN - 0008-5472, 1538-7445 UR - https://cancerres.aacrjournals.org/content/early/2021/02/01/0008-5472.CAN-20-1037 Y2 - 2021/02/03/19:14:59 ER -