TY - JOUR TI - Novel carbon film induces precocious calcium oscillation to promote neuronal cell maturation AU - Ludwig, Anastasia AU - Kesaf, Sebnem AU - Heikkinen, Joonas J. AU - Sukhanova, Tatiana AU - Khakipoor, Shokoufeh AU - Molinari, Florence AU - Pellegrino, Christophe AU - Kim, Sung I. AU - Han, Jeon G. AU - Huttunen, Henri J. AU - Lauri, Sari E. AU - Franssila, Sami AU - Jokinen, Ville AU - Rivera, Claudio T2 - Scientific Reports AB - Different types of carbon materials are biocompatible with neural cells and can promote maturation. The mechanism of this effect is not clear. Here we have tested the capacity of a carbon material composed of amorphous sp3 carbon backbone, embedded with a percolating network of sp2 carbon domains to sustain neuronal cultures. We found that cortical neurons survive and develop faster on this novel carbon material. After 3 days in culture, there is a precocious increase in the frequency of neuronal activity and in the expression of maturation marker KCC2 on carbon films as compared to a commonly used glass surface. Accelerated development is accompanied by a dramatic increase in neuronal dendrite arborization. The mechanism for the precocious maturation involves the activation of intracellular calcium oscillations by the carbon material already after 1 day in culture. Carbon-induced oscillations are independent of network activity and reflect intrinsic spontaneous activation of developing neurons. Thus, these results reveal a novel mechanism for carbon material-induced neuronal survival and maturation. DA - 2020/10/19/ PY - 2020 DO - 10.1038/s41598-020-74535-6 DP - www.nature.com VL - 10 IS - 1 SP - 17661 LA - en SN - 2045-2322 UR - https://www.nature.com/articles/s41598-020-74535-6 Y2 - 2020/10/19/20:25:53 ER -