TY - JOUR TI - Protection of β-pancreatic cells from dysfunctionality of insulin using vitexin by apoptosis of INS-1 cells AU - Zhang, Li AU - Shi, Lianfeng AU - Han, Juanjuan AU - Li, Zhenzuo T2 - Archives of Physiology and Biochemistry AB - Aims This study was performed to explore the possible beneficial effects of vitexin on high glucose (HG)-induced cytotoxicity in pancreatic β-cells.Methods INS-1 pancreatic β-cell line has used this study. HG-induced (33 Mm) exposed INS-1 cell death; the apoptosis INS-1 cells treated vitexin 10, 20, 40, and 80 µg/mL for 24 hours. The anti-apoptosis properties were evaluated by MTT assay, glucose-stimulated insulin secretion assay, biochemical assay, annexin-V-FITC staining and western blot analysis.Results These findings demonstrate that vitexin treatment improved the HG-exposure, reduced the INS-1 cell viability and significantly enhanced glucose-stimulated insulin secretion in a dose-dependent manner. The antioxidant studies revealed that vitexin treatment significantly decreased lipid peroxidation and reactive oxygen species and increased antioxidant level of INS-1 cell line in 24 hrs. The findings of the study suggested that in the vitexin treatment group, pancreatic apoptosis and Bax protein expression reduced significantly. At the same time, Bcl-2 protein expression increased, and NF-κB protein in HG-induced INS-cells was inhibited.Conclusion Therefore, our results suggest that vitexin can be successfully used to regulate the expression of Bcl-2 family proteins, reduce lipid peroxidation and to improve the secretion of antioxidants in pancreatic β-cell lines. DA - 2021/04/09/ PY - 2021 DO - 10.1080/13813455.2021.1910714 DP - Taylor and Francis+NEJM VL - 0 IS - 0 SP - 1 EP - 8 SN - 1381-3455 UR - https://doi.org/10.1080/13813455.2021.1910714 Y2 - 2021/04/13/16:47:03 KW - NF-κB pancreatic β-cells KW - Vitexin KW - anti-apoptosis KW - antioxidant KW - high glucose KW - insulin secretion ER -