TY - JOUR TI - Long-term self-renewing stem cells in the adult mouse hippocampus identified by intravital imaging AU - Bottes, Sara AU - Jaeger, Baptiste N. AU - Pilz, Gregor-Alexander AU - Jörg, David J. AU - Cole, John Darby AU - Kruse, Merit AU - Harris, Lachlan AU - Korobeynyk, Vladislav I. AU - Mallona, Izaskun AU - Helmchen, Fritjof AU - Guillemot, François AU - Simons, Benjamin D. AU - Jessberger, Sebastian T2 - Nature Neuroscience AB - Neural stem cells (NSCs) generate neurons throughout life in the mammalian hippocampus. However, the potential for long-term self-renewal of individual NSCs within the adult brain remains unclear. We used two-photon microscopy and followed NSCs that were genetically labeled through conditional recombination driven by the regulatory elements of the stem cell-expressed genes GLI family zinc finger 1 (Gli1) or achaete-scute homolog 1 (Ascl1). Through intravital imaging of NSCs and their progeny, we identify a population of Gli1-targeted NSCs showing long-term self-renewal in the adult hippocampus. In contrast, once activated, Ascl1-targeted NSCs undergo limited proliferative activity before they become exhausted. Using single-cell RNA sequencing, we show that Gli1- and Ascl1-targeted cells have highly similar yet distinct transcriptional profiles, supporting the existence of heterogeneous NSC populations with diverse behavioral properties. Thus, we here identify long-term self-renewing NSCs that contribute to the generation of new neurons in the adult hippocampus. DA - 2020/12/21/ PY - 2020 DO - 10.1038/s41593-020-00759-4 DP - www.nature.com SP - 1 EP - 9 LA - en SN - 1546-1726 UR - https://www.nature.com/articles/s41593-020-00759-4 Y2 - 2021/01/04/21:23:38 ER -