TY - JOUR TI - MicroRNA-488 inhibits proliferation and motility of tumor cells via downregulating FSCN1, modulated by Notch3 in breast carcinomas AU - Wu, Yang AU - Yuan, Ming-Heng AU - Wu, Hua-Tao AU - Chen, Wen-Jia AU - Zhang, Man-Li AU - Ye, Qian-Qian AU - Liu, Jing AU - Zhang, Guo-Jun T2 - Cell Death & Disease AB - As important modulators in multiple physiological processes, microRNAs (miRNAs) have been reported in various malignant tumors, including breast cancer. The current study investigated the function of a new tumor suppressor microRNA, miR-488, and its molecular mechanism of metastasis in breast cancers. CCK8 and transwell assays revealed that the upregulated miR-488 level significantly inhibited the proliferation and migration of breast cancer cells. As a potential downstream gene, the mRNA and protein level of FSCN1 was suppressed by increased miR-488 and vice versa. Luciferase assay showed that miR-488 directly bind to the 3′UTR of FSCN1 and suppressed the translation process of FSCN1. The promoter region of miR-488 was directly bound by Notch3 and promoted the expression of miR-488 transcriptionally. Immunohistochemistry results revealed that in patients with breast cancer, the expression of Notch3 and were negatively correlated with the FSCN1 levels significantly. Therefore, the current finding predicted miR-488 as a tumor suppressor molecule in breast cancer, and demonstrated that Notch3/miR-488/FSCN1 axis is established and involved in regulating the metastasis of breast cancers, providing novel therapeutic targets for patients with breast cancers. DA - 2020/10/24/ PY - 2020 DO - 10.1038/s41419-020-03121-5 DP - www.nature.com VL - 11 IS - 10 SP - 1 EP - 13 LA - en SN - 2041-4889 UR - https://www.nature.com/articles/s41419-020-03121-5 Y2 - 2020/10/26/22:15:32 ER -