TY - JOUR TI - A High-Dimensional Window into the Micro-Environment of Triple Negative Breast Cancer AU - Nederlof, Iris AU - Horlings, Hugo M. AU - Curtis, Christina AU - Kok, Marleen T2 - Cancers AB - Providing effective personalized immunotherapy for triple negative breast cancer (TNBC) patients requires a detailed understanding of the composition of the tumor microenvironment. Both the tumor cell and non-tumor components of TNBC can exhibit tremendous heterogeneity in individual patients and change over time. Delineating cellular phenotypes and spatial topographies associated with distinct immunological states and the impact of chemotherapy will be necessary to optimally time immunotherapy. The clinical successes in immunotherapy have intensified research on the tumor microenvironment, aided by a plethora of high-dimensional technologies to define cellular phenotypes. These high-dimensional technologies include, but are not limited to, single cell RNA sequencing, spatial transcriptomics, T cell repertoire analyses, advanced flow cytometry, imaging mass cytometry, and their integration. In this review, we discuss the cellular phenotypes and spatial patterns of the lymphoid-, myeloid-, and stromal cells in the TNBC microenvironment and the potential value of mapping these features onto tumor cell genotypes. DA - 2021/01// PY - 2021 DO - 10.3390/cancers13020316 DP - www.mdpi.com VL - 13 IS - 2 SP - 316 LA - en UR - https://www.mdpi.com/2072-6694/13/2/316 Y2 - 2021/01/21/00:01:53 KW - breast cancer KW - immune profiling KW - single cell KW - spatial profiling KW - tumor evolution ER -