TY - JOUR TI - Integrative transcriptomic analysis for linking acute stress responses to squamous cell carcinoma development AU - Nguyen, Tran N. AU - Rajapakshe, Kimal AU - Nicholas, Courtney AU - Tordesillas, Leticia AU - Ehli, Erik A. AU - Davis, Christel M. AU - Coarfa, Cristian AU - Flores, Elsa R. AU - Dickinson, Sally E. AU - Curiel-Lewandrowski, Clara AU - Tsai, Kenneth Y. T2 - Scientific Reports AB - Cutaneous squamous cell carcinoma (cuSCC) is the second most common skin cancer and commonly arises in chronically UV-exposed skin or chronic wounds. Since UV exposure and chronic wounds are the two most prominent environmental factors that lead to cuSCC initiation, we undertook this study to test whether more acute molecular responses to UV and wounding overlapped with molecular signatures of cuSCC. We reasoned that transcriptional signatures in common between acutely UV-exposed skin, wounded skin, and cuSCC tumors, might enable us to identify important pathways contributing to cuSCC. We performed transcriptomic analysis on acutely UV-exposed human skin and integrated those findings with datasets from wounded skin and our transcriptomic data on cuSCC using functional pair analysis, GSEA, and pathway analysis. Integrated analyses revealed significant overlap between these three datasets, thus highlighting deep molecular similarities these biological processes, and we identified Oncostatin M (OSM) as a potential common upstream driver. Expression of OSM and its downstream targets correlated with poorer overall survival in head and neck SCC patients. In vitro, OSM promoted invasiveness of keratinocytes and cuSCC cells and suppressed apoptosis of irradiated keratinocytes. Together, these results support the concept of using an integrated, biologically-informed approach to identify potential promoters of tumorigenesis. DA - 2020/10/14/ PY - 2020 DO - 10.1038/s41598-020-74051-7 DP - www.nature.com VL - 10 IS - 1 SP - 17209 LA - en SN - 2045-2322 UR - https://www.nature.com/articles/s41598-020-74051-7 Y2 - 2020/10/14/21:12:55 ER -