Home Newsletters Hematopoiesis News Enhanced Collection of Phenotypic and Engrafting Human Cord Blood Hematopoietic Stem Cells...

Enhanced Collection of Phenotypic and Engrafting Human Cord Blood Hematopoietic Stem Cells at 4°C

0
Researchers demonstrated that collecting and processing of human cord blood at 4°C within minutes of the baby’s birth results in significantly enhanced numbers of rigorously defined phenotypic hematopoietic stem cell and self‐renewing NSG immune‐deficient mouse engrafting and SCID‐repopulating cells.
[Stem Cells]
6445212 {1254621:7XMPZLN5} apa 50 1 150819 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-24ffd7cfc0ba6a9082d7a3708ef9ccb5%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%227XMPZLN5%22%2C%22library%22%3A%7B%22id%22%3A1254621%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Broxmeyer%20et%20al.%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBroxmeyer%2C%20H.%20E.%2C%20Cooper%2C%20S.%2C%20%26amp%3B%20Capitano%2C%20M.%20L.%20%28n.d.%29.%20Enhanced%20Collection%20of%20Phenotypic%20and%20Engrafting%20Human%20Cord%20Blood%20Hematopoietic%20Stem%20Cells%20at%204%26%23xB0%3BC.%20%3Ci%3ESTEM%20CELLS%3C%5C%2Fi%3E%2C%20%3Ci%3En%5C%2Fa%3C%5C%2Fi%3E%28n%5C%2Fa%29.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fstem.3243%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1002%5C%2Fstem.3243%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D1254621%26amp%3Bitem_key%3D7XMPZLN5%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Enhanced%20Collection%20of%20Phenotypic%20and%20Engrafting%20Human%20Cord%20Blood%20Hematopoietic%20Stem%20Cells%20at%204%5Cu00b0C%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hal%20E.%22%2C%22lastName%22%3A%22Broxmeyer%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Scott%22%2C%22lastName%22%3A%22Cooper%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maegan%20L.%22%2C%22lastName%22%3A%22Capitano%22%7D%5D%2C%22abstractNote%22%3A%22Hematopoietic%20stem%20cell%20%28HSC%29%20numbers%20collected%20in%20cord%20blood%20%28CB%29%20at%20the%20birth%20of%20a%20baby%20is%20a%20limiting%20factor%20for%20efficacious%20use%20of%20CB%20in%20hematopoietic%20cell%20transplantation%20%28HCT%29.%20We%20now%20demonstrate%20that%20collecting%20and%20processing%20of%20human%20CB%20at%204%5Cu00b0C%20within%20minutes%20of%20the%20baby%27s%20birth%20results%20in%20significantly%20enhanced%20numbers%20of%20rigorously%20defined%20phenotypic%20HSC%20and%20self-renewing%20NSG%20immune-deficient%20mouse%20engrafting%20and%20SCID-repopulating%20cells.%20This%20was%20associated%20with%20decreased%20numbers%20of%20hematopoietic%20progenitor%20cells%20%28HPC%29%2C%20as%20noted%20previously%20for%20hypoxia%20collected%5C%2Fprocessed%20cells%20blocking%20ambient%20air%20induced%20differentiation%20of%20HSC%20to%20HPC.%20We%20have%20thus%20defined%20a%20simple%2C%20cost-effective%2C%20means%20to%20collect%20increased%20numbers%20of%20CB%20HSC%2C%20of%20potential%20use%20for%20clinical%20CB%20HCT.%20%5Cu00a9%20AlphaMed%20Press%202020%20Significance%20Statement%20Numbers%20of%20collected%20cord%20blood%20hematopoietic%20stem%20cells%20at%20the%20birth%20of%20a%20baby%20is%20a%20limiting%20factor%20in%20use%20of%20cord%20blood%20for%20hematopoietic%20cell%20transplantation.%20We%20now%20demonstrate%2C%20by%20collecting%20and%20processing%20cord%20blood%20at%204%5Cu00b0C%2C%20that%20we%20can%20attain%20significantly%20enhanced%20numbers%20of%20cord%20blood%20hematopoietic%20stem%20cells%20for%20potential%20use%20in%20clinical%20cord%20blood%20hematopoietic%20cell%20transplantation%2C%20in%20a%20simple%20and%20cost-effective%20manner.%22%2C%22date%22%3A%22%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1002%5C%2Fstem.3243%22%2C%22ISSN%22%3A%221549-4918%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fstemcellsjournals.onlinelibrary.wiley.com%5C%2Fdoi%5C%2Fabs%5C%2F10.1002%5C%2Fstem.3243%22%2C%22deleted%22%3A1%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222020-06-17T16%3A00%3A45Z%22%7D%7D%5D%7D
Broxmeyer, H. E., Cooper, S., & Capitano, M. L. (n.d.). Enhanced Collection of Phenotypic and Engrafting Human Cord Blood Hematopoietic Stem Cells at 4°C. STEM CELLS, n/a(n/a). https://doi.org/10.1002/stem.3243 Cite
Abstract
Exit mobile version