Home Newsletters Hepatic Cell News Vitamin E Pretreated Wharton’s Jelly-Derived Mesenchymal Stem Cells Attenuate CCl4-Induced Hepatocyte Injury...

Vitamin E Pretreated Wharton’s Jelly-Derived Mesenchymal Stem Cells Attenuate CCl4-Induced Hepatocyte Injury In Vitro and Liver Fibrosis In Vivo

0
The authors used rat liver-derived hepatocytes injured by CCl4 treatment in co-culture system with Vitamin E pretreated-Wharton’s jelly-derived MSCs (WJMSCs) to evaluate the hepatoprotective effect of vitamin E-WJMSCs.
[Biochemical Pharmacology]
6445212 {6445212:QQ8SQSZ6} apa 50 1 159352 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-3bc3cfbd1a7f1843e60d0bc2d7cbe8f4%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22QQ8SQSZ6%22%2C%22library%22%3A%7B%22id%22%3A6445212%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Tayyab%20Baig%20et%20al.%22%2C%22parsedDate%22%3A%222021-02-20%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ETayyab%20Baig%2C%20M.%2C%20Ghufran%2C%20H.%2C%20Mehmood%2C%20A.%2C%20Azam%2C%20M.%2C%20Humayun%2C%20S.%2C%20%26amp%3B%20Riazuddin%2C%20S.%20%282021%29.%20Vitamin%20E%20pretreated%20Wharton%26%23x2019%3Bs%20jelly-derived%20mesenchymal%20stem%20cells%20attenuate%20CCl4-induced%20hepatocyte%20injury%20in%20vitro%20and%20liver%20fibrosis%20in%20vivo.%20%3Ci%3EBiochemical%20Pharmacology%3C%5C%2Fi%3E%2C%20114480.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.bcp.2021.114480%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.bcp.2021.114480%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445212%26amp%3Bitem_key%3DQQ8SQSZ6%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Vitamin%20E%20pretreated%20Wharton%5Cu2019s%20jelly-derived%20mesenchymal%20stem%20cells%20attenuate%20CCl4-induced%20hepatocyte%20injury%20in%20vitro%20and%20liver%20fibrosis%20in%20vivo%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%22%2C%22lastName%22%3A%22Tayyab%20Baig%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hafiz%22%2C%22lastName%22%3A%22Ghufran%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Azra%22%2C%22lastName%22%3A%22Mehmood%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maryam%22%2C%22lastName%22%3A%22Azam%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shamsa%22%2C%22lastName%22%3A%22Humayun%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sheikh%22%2C%22lastName%22%3A%22Riazuddin%22%7D%5D%2C%22abstractNote%22%3A%22Oxidative%20microenvironment%20in%20fibrotic%20liver%20alleviates%20the%20efficacious%20outcome%20of%20mesenchymal%20stem%20cells%20%28MSCs%29-based%20cell%20therapy.%20Recent%20evidence%20suggests%20that%20pharmacological%20pretreatment%20is%20a%20rational%20approach%20to%20harness%20the%20MSCs%20with%20higher%20therapeutic%20potential.%20Here%2C%20we%20investigated%20whether%20Vitamin%20E%20pretreatment%20can%20boost%20the%20antifibrotic%20effects%20of%20Wharton%5Cu2019s%20jelly-derived%20MSCs%20%28WJMSCs%29.%20We%20used%20rat%20liver-derived%20hepatocytes%20injured%20by%20CCl4%20treatment%20in%20co-culture%20system%20with%20Vitamin%20E%20pretreated-WJMSCs%20%28Vit%20E-WJMSCs%29%20to%20evaluate%20the%20hepatoprotective%20effect%20of%20Vit%20E-WJMSCs.%20After%2024%20h%20of%20co-culturing%2C%20we%20found%20that%20Vit%20E-WJMSCs%20rescued%20injured%20hepatocytes%20as%20hepatocyte%20injury-associated%20medium%20%28AST%2C%20ALT%2C%20and%20ALP%29%20and%20mRNA%20%28Cyp2e1%2C%20Hif1-%5Cu03b1%2C%20and%20Il-1%5Cu03b2%29%20markers%20reduced%20to%20normal%20levels.%20Subsequently%2C%20CCl4-induced%20liver%20fibrosis%20rat%20models%20were%20employed%20to%20examine%20the%20antifibrotic%20potential%20of%20Vit%20E-WJMSCs.%20After%201%20month%20of%20cell%20transplantation%2C%20it%20was%20revealed%20that%20Vit%20E-WJMSCs%20transplantation%20ceased%20fibrotic%20progression%2C%20as%20evident%20by%20improved%20hepatic%20architecture%20and%20functions%2C%20more%20significantly%20in%20comparison%20to%20na%5Cu00efve%20WJMSCs.%20In%20addition%2C%20Vit%20E-WJMSCs%20transplantation%20decreased%20the%20expressions%20of%20fibrosis-associated%20gene%20%28Tgf-%5Cu03b21%2C%20%5Cu03b1-Sma%2C%20and%20Col1%5Cu03b11%29%20markers%20in%20the%20liver%20parenchyma.%20Intriguingly%2C%20the%20results%20of%20tracing%20experiments%20discovered%20that%20more%20WJMSCs%20engrafted%20in%20the%20Vit%20E-WJMSCs%20treated%20rat%20livers%20compared%20to%20na%5Cu00efve%20WJMSCs%20treated%20livers.%20These%20findings%20implicate%20that%20pretreatment%20of%20WJMSCs%20with%20Vitamin%20E%20improves%20their%20tolerance%20to%20hostile%20niche%20of%20fibrotic%20liver%3B%20thereby%20further%20enhancing%20their%20efficacy%20for%20hepatic%20fibrosis.%22%2C%22date%22%3A%22February%2020%2C%202021%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.bcp.2021.114480%22%2C%22ISSN%22%3A%220006-2952%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.sciencedirect.com%5C%2Fscience%5C%2Farticle%5C%2Fpii%5C%2FS0006295221000769%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-02-23T18%3A55%3A38Z%22%7D%7D%5D%7D
Tayyab Baig, M., Ghufran, H., Mehmood, A., Azam, M., Humayun, S., & Riazuddin, S. (2021). Vitamin E pretreated Wharton’s jelly-derived mesenchymal stem cells attenuate CCl4-induced hepatocyte injury in vitro and liver fibrosis in vivo. Biochemical Pharmacology, 114480. https://doi.org/10.1016/j.bcp.2021.114480 Cite
Full ArticleGraphical Abstract
Exit mobile version