Home Newsletters Pulmonary Cell News Cellular Retinol Binding Protein 1 Transfection Reduces Proliferation and AKT-Related Gene Expression...

Cellular Retinol Binding Protein 1 Transfection Reduces Proliferation and AKT-Related Gene Expression in H460 Non-Small Lung Cancer Cells

0
Scientists investigated the effect of cellular retinol binding protein-1 (CRBP-1) transfection in the H460 human non-small cell lung cancer cell line, normally not expressing CRBP-1.
[Molecular Biology Reports]
1254621 {1254621:TNNJKYAR} apa 50 1 153989 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-4f18218c194fa10706c4fc15511eb80d%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22TNNJKYAR%22%2C%22library%22%3A%7B%22id%22%3A1254621%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Ferlosio%20et%20al.%22%2C%22parsedDate%22%3A%222020-09-09%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EFerlosio%2C%20A.%2C%20Doldo%2C%20E.%2C%20Agostinelli%2C%20S.%2C%20Costanza%2C%20G.%2C%20Centofanti%2C%20F.%2C%20Sidoni%2C%20A.%2C%20%26amp%3B%20Orlandi%2C%20A.%20%282020%29.%20Cellular%20retinol%20binding%20protein%201%20transfection%20reduces%20proliferation%20and%20AKT-related%20gene%20expression%20in%20H460%20non-small%20lung%20cancer%20cells.%20%3Ci%3EMolecular%20Biology%20Reports%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs11033-020-05744-5%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs11033-020-05744-5%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D1254621%26amp%3Bitem_key%3DTNNJKYAR%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Cellular%20retinol%20binding%20protein%201%20transfection%20reduces%20proliferation%20and%20AKT-related%20gene%20expression%20in%20H460%20non-small%20lung%20cancer%20cells%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Amedeo%22%2C%22lastName%22%3A%22Ferlosio%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Elena%22%2C%22lastName%22%3A%22Doldo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sara%22%2C%22lastName%22%3A%22Agostinelli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gaetana%22%2C%22lastName%22%3A%22Costanza%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Federica%22%2C%22lastName%22%3A%22Centofanti%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Angelo%22%2C%22lastName%22%3A%22Sidoni%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Augusto%22%2C%22lastName%22%3A%22Orlandi%22%7D%5D%2C%22abstractNote%22%3A%22In%20recent%20years%2C%20new%20treatments%20with%20novel%20action%20mechanisms%20have%20been%20explored%20for%20advanced%20non-small%20cell%20lung%20cancer%20%28NSCLC%29.%20Retinoids%20promote%20cancer%20cell%20differentiation%20and%20death%20and%20their%20trafficking%20and%20action%20is%20mediated%20from%20specific%20cytoplasmic%20and%20nuclear%20receptors%2C%20respectively.%20The%20purpose%20of%20this%20study%20was%20to%20investigate%20the%20effect%20of%20Cellular%20retinol%20binding%20protein-1%20%28CRBP-1%29%20transfection%20in%20H460%20human%20NSCLC%20cell%20line%2C%20normally%20not%20expressing%20CRBP-1.%5Cu00a0H460%20cells%20were%20transfected%20by%20using%20a%20vector%20pTargeT%20Mammalian%20expression%20system%20carrying%20the%20whole%20sequence%20of%20CRBP-1%20gene.%20For%20proliferation%20and%20apoptosis%20studies%2C%20cells%20were%20treated%20with%20different%20concentrations%20of%20all-trans%20Retinoic%20Acid%20%28atRA%29%20and%20retinol.%20AKT-related%20gene%20expression%20was%20analyzed%20by%20using%20western%20blot%20and%20Signosis%20array%20and%20results%20analysed%20by%20one-way%20analysis%20of%20variance%20%28ANOVA%29%20or%20by%20t-student%20test.%5Cu00a0CRBP-1%2B%20showed%20reduced%20proliferation%20and%20viability%20in%20basal%20condition%20and%20after%20atRA%20treatment%20when%20compared%20to%20empty-transfected%20H460%20cells.%20Reduced%20proliferation%20in%20CRBP-1%2B%20H460%20cells%20associated%20to%20the%20down-regulation%20of%20pAKT%5C%2FpERK%5C%2FpEGFR-related%20genes.%20In%20particular%2C%20gene%20array%20documented%20the%20down-regulation%20of%20AKT%20and%20Stat-3-related%20genes%2C%20including%20M-Tor%2C%20Akt1%2C%20Akt2%2C%20Akt3%2C%20Foxo1%2C%20p27%2C%20Jun.%5Cu00a0Restoration%20of%20CRBP-1%20expression%20in%20H460%20cells%20reduced%20proliferation%20and%20viability%20in%20both%20basal%20condition%20and%20after%20atRA%20treatment%2C%20likely%20by%20down-regulating%20AKT-related%20gene%20level.%20Further%20studies%20are%20needed%20to%20better%20clarify%20how%20those%20CRBP-1-related%20intracellular%20pathways%20contribute%20to%20counteract%20NSCLC%20progression%20in%20order%20to%20suggest%20a%20potential%20tool%20to%20improve%20efficacy%20of%20retinoid%20anti%20lung%20cancer%20adjuvant%20therapy.%22%2C%22date%22%3A%222020-09-09%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1007%5C%2Fs11033-020-05744-5%22%2C%22ISSN%22%3A%221573-4978%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1007%5C%2Fs11033-020-05744-5%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222020-09-11T18%3A02%3A44Z%22%7D%7D%5D%7D
Ferlosio, A., Doldo, E., Agostinelli, S., Costanza, G., Centofanti, F., Sidoni, A., & Orlandi, A. (2020). Cellular retinol binding protein 1 transfection reduces proliferation and AKT-related gene expression in H460 non-small lung cancer cells. Molecular Biology Reports. https://doi.org/10.1007/s11033-020-05744-5 Cite
Full Article
Exit mobile version