Human intestinal stem cell-derived enteroids did not support virus replication from every HuNoV-positive stool sample, which led the authors to test and optimize new medium conditions, identify characteristics of stool samples that allow replication, and evaluate consistency of replication over time.
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Ettayebi, K., Tenge, V. R., Cortes-Penfield, N. W., Crawford, S. E., Neill, F. H., Zeng, X.-L., Yu, X., Ayyar, B. V., Burrin, D., Ramani, S., Atmar, R. L., & Estes, M. K. (2021). New Insights and Enhanced Human Norovirus Cultivation in Human Intestinal Enteroids. MSphere, 6(1). https://doi.org/10.1128/mSphere.01136-20 Cite
