Home Newsletters Neural Cell News Optogenetic Manipulation of Cellular Communication Using Engineered Myosin Motors

Optogenetic Manipulation of Cellular Communication Using Engineered Myosin Motors

0
Researchers presented a versatile optogenetic toolbox of artificial multi-headed myosin motors that could move bidirectionally within long cellular extensions and allowed for the selective transport of GFP-tagged cargo with light.
[Nature Cell Biology]
6807162 {6807162:2NNWAGH6} apa 50 1 158577 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-736b609ae3eddfd7ba60a8c6a2475f0c%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%222NNWAGH6%22%2C%22library%22%3A%7B%22id%22%3A6807162%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Zhang%20et%20al.%22%2C%22parsedDate%22%3A%222021-02-01%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EZhang%2C%20Z.%2C%20Denans%2C%20N.%2C%20Liu%2C%20Y.%2C%20Zhulyn%2C%20O.%2C%20Rosenblatt%2C%20H.%20D.%2C%20Wernig%2C%20M.%2C%20%26amp%3B%20Barna%2C%20M.%20%282021%29.%20Optogenetic%20manipulation%20of%20cellular%20communication%20using%20engineered%20myosin%20motors.%20%3Ci%3ENature%20Cell%20Biology%3C%5C%2Fi%3E%2C%201%26%23x2013%3B11.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41556-020-00625-2%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41556-020-00625-2%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6807162%26amp%3Bitem_key%3D2NNWAGH6%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Optogenetic%20manipulation%20of%20cellular%20communication%20using%20engineered%20myosin%20motors%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zijian%22%2C%22lastName%22%3A%22Zhang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicolas%22%2C%22lastName%22%3A%22Denans%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yingfei%22%2C%22lastName%22%3A%22Liu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olena%22%2C%22lastName%22%3A%22Zhulyn%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hannah%20D.%22%2C%22lastName%22%3A%22Rosenblatt%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Marius%22%2C%22lastName%22%3A%22Wernig%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%22%2C%22lastName%22%3A%22Barna%22%7D%5D%2C%22abstractNote%22%3A%22Cells%20achieve%20highly%20efficient%20and%20accurate%20communication%20through%20cellular%20projections%20such%20as%20neurites%20and%20filopodia%2C%20yet%20there%20is%20a%20lack%20of%20genetically%20encoded%20tools%20that%20can%20selectively%20manipulate%20their%20composition%20and%20dynamics.%20Here%2C%20we%20present%20a%20versatile%20optogenetic%20toolbox%20of%20artificial%20multi-headed%20myosin%20motors%20that%20can%20move%20bidirectionally%20within%20long%20cellular%20extensions%20and%20allow%20for%20the%20selective%20transport%20of%20GFP-tagged%20cargo%20with%20light.%20Utilizing%20these%20engineered%20motors%2C%20we%20could%20transport%20bulky%20transmembrane%20receptors%20and%20organelles%20as%20well%20as%20actin%20remodellers%20to%20control%20the%20dynamics%20of%20both%20filopodia%20and%20neurites.%20Using%20an%20optimized%20in%20vivo%20imaging%20scheme%2C%20we%20further%20demonstrate%20that%2C%20upon%20limb%20amputation%20in%20axolotls%2C%20a%20complex%20array%20of%20filopodial%20extensions%20is%20formed.%20We%20selectively%20modulated%20these%20filopodial%20extensions%20and%20showed%20that%20they%20re-establish%20a%20Sonic%20Hedgehog%20signalling%20gradient%20during%20regeneration.%20Considering%20the%20ubiquitous%20existence%20of%20actin-based%20extensions%2C%20this%20toolbox%20shows%20the%20potential%20to%20manipulate%20cellular%20communication%20with%20unprecedented%20accuracy.%22%2C%22date%22%3A%222021-02-01%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41556-020-00625-2%22%2C%22ISSN%22%3A%221476-4679%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41556-020-00625-2%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-02-01T21%3A54%3A28Z%22%7D%7D%5D%7D
Zhang, Z., Denans, N., Liu, Y., Zhulyn, O., Rosenblatt, H. D., Wernig, M., & Barna, M. (2021). Optogenetic manipulation of cellular communication using engineered myosin motors. Nature Cell Biology, 1–11. https://doi.org/10.1038/s41556-020-00625-2 Cite
Abstract
Exit mobile version