When grown under the same stem cell enriching conditions, SHH subgroup medulloblastoma cell lines established tight, highly reproducible 3D spheroids that could be maintained for weeks in culture and formed pathophysiological oxygen gradients.
[Scientific Reports]
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Roper, S. J., Linke, F., Scotting, P. J., & Coyle, B. (2021). 3D spheroid models of paediatric SHH medulloblastoma mimic tumour biology, drug response and metastatic dissemination. Scientific Reports, 11(1), 4259. https://doi.org/10.1038/s41598-021-83809-6 Cite
