Using patient-derived iPSCs and an in vitro artificial thymic organoid system as a model, investigators demonstrate that gene editing rescued the progressive T cell differentiation potential of RAG2-deficient cells to normal levels, with generation of a diversified T cell repertoire.
[Journal of Clinical Immunology]
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Gardner, C. L., Pavel-Dinu, M., Dobbs, K., Bosticardo, M., Reardon, P. K., Lack, J., DeRavin, S. S., Le, K., Bello, E., Pala, F., Delmonte, O. M., Malech, H., Montel-Hagan, A., Crooks, G., Acuto, O., Porteus, M. H., & Notarangelo, L. D. (2021). Gene Editing Rescues In vitro T Cell Development of RAG2-Deficient Induced Pluripotent Stem Cells in an Artificial Thymic Organoid System. Journal of Clinical Immunology. https://doi.org/10.1007/s10875-021-00989-6 Cite
