Investigators found that GPR126 had higher expression in most colorectal cancer cell lines than in normal colon epithelial cell lines, and higher expression levels in colorectal cancer tissues than in normal adjacent colon tissues.
[Cancer Science]
6445212
{6445212:LVKZGIB8}
apa
50
1
159783
https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-887480b0759d6eb5e1cff79838b40f5f%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22LVKZGIB8%22%2C%22library%22%3A%7B%22id%22%3A6445212%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Cui%20et%20al.%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ECui%2C%20H.%2C%20Yu%2C%20W.%2C%20Yu%2C%20M.%2C%20Luo%2C%20Y.%2C%20Yang%2C%20M.%2C%20Cong%2C%20R.%2C%20Chu%2C%20X.%2C%20Gao%2C%20G.%2C%20%26amp%3B%20Zhong%2C%20M.%20%28n.d.%29.%20GPR126%20regulates%20colorectal%20cancer%20cell%20proliferation%20by%20mediating%20HDAC2%20and%20GLI2%20expression.%20%3Ci%3ECancer%20Science%3C%5C%2Fi%3E%2C%20%3Ci%3En%5C%2Fa%3C%5C%2Fi%3E%28n%5C%2Fa%29.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2Fhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fcas.14868%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2Fhttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fcas.14868%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445212%26amp%3Bitem_key%3DLVKZGIB8%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22GPR126%20regulates%20colorectal%20cancer%20cell%20proliferation%20by%20mediating%20HDAC2%20and%20GLI2%20expression%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hengxiang%22%2C%22lastName%22%3A%22Cui%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Wenjie%22%2C%22lastName%22%3A%22Yu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Minhao%22%2C%22lastName%22%3A%22Yu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yang%22%2C%22lastName%22%3A%22Luo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Mingming%22%2C%22lastName%22%3A%22Yang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ruochen%22%2C%22lastName%22%3A%22Cong%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xin%22%2C%22lastName%22%3A%22Chu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ganglong%22%2C%22lastName%22%3A%22Gao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ming%22%2C%22lastName%22%3A%22Zhong%22%7D%5D%2C%22abstractNote%22%3A%22The%20G-protein-coupled%20receptor%20126%20%28GPR126%29%20may%20play%20an%20important%20role%20in%20tumor%20development.%20although%20its%20role%20remains%20poorly%20understood.%20We%20found%20that%20GPR126%20had%20higher%20expression%20in%20most%20colorectal%20cancer%20cell%20lines%20than%20in%20normal%20colon%20epithelial%20cell%20lines%2C%20and%20higher%20expression%20levels%20in%20colorectal%20cancer%20tissues%20than%20in%20normal%20adjacent%20colon%20tissues.%20GPR126%20knockdown%20induced%20by%20shRNA%20inhibited%20cell%20viability%20and%20colony%20formation%20in%20HT-29%2C%20HCT116%2C%20and%20LoVo%20cells%2C%20decreased%20BrdU%20incorporation%20into%20newly%20synthesized%20proliferating%20HT-29%20cells%2C%20led%20to%20an%20arrest%20of%20cell%20cycle%20progression%20at%20the%20G1%20phase%20in%20HCT-116%20and%20HT-29%20cells%2C%20and%20suppressed%20tumorigenesis%20of%20HT-29%2C%20HCT116%2C%20and%20LoVo%20cells%20in%20nude%20mouse%20xenograft%20models.%20GPR126%20knockdown%20engendered%20decreased%20transcription%20and%20translation%20of%20histone%20deacetylase%202%20%28HDAC2%29%2C%20previously%20implicated%20in%20the%20activation%20of%20GLI1%20and%20GLI2%20in%20the%20Hedgehog%20signaling%20pathway.%20Ectopic%20expression%20of%20HDAC2%20in%20GPR126-silenced%20cells%20restored%20cell%20viability%20and%20proliferation%2C%20GLI2%20luciferase%20reporter%20activity%2C%20partially%20recovered%20GLI2%20expression%2C%20and%20reduced%20the%20cell%20cycle%20arrest.%20HDAC2%20regulated%20GLI2%20expression%20and%2C%20along%20with%20GLI2%2C%20it%20bound%20to%20the%20PTCH1%20promoter%2C%20as%20evidenced%20by%20a%20chip%20assay%20with%20HT-29%20cells.%20Purmorphamine%2C%20a%20hedgehog%20agonist%2C%20largely%20restored%20the%20cell%20viability%20and%20expression%20of%20GLI2%20proteins%20in%20GPR126-silenced%20HT-29%20cells%2C%20whereas%20GANT61%2C%20a%20hedgehog%20inhibitor%2C%20further%20enhanced%20the%20GPR126%20knockdown-induced%20inhibitory%20effects.%20Our%20findings%20demonstrate%20that%20GPR126%20regulates%20colorectal%20cancer%20cell%20proliferation%20by%20mediating%20the%20expression%20of%20HDAC2%20and%20GLI2%3B%20therefore%2C%20it%20may%20represent%20a%20suitable%20therapeutic%20target%20for%20colorectal%20cancer%20treatment.%22%2C%22date%22%3A%22%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1111%5C%2Fcas.14868%22%2C%22ISSN%22%3A%221349-7006%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fonlinelibrary.wiley.com%5C%2Fdoi%5C%2Fabs%5C%2F10.1111%5C%2Fcas.14868%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-03-04T23%3A07%3A37Z%22%7D%7D%5D%7D
Cui, H., Yu, W., Yu, M., Luo, Y., Yang, M., Cong, R., Chu, X., Gao, G., & Zhong, M. (n.d.). GPR126 regulates colorectal cancer cell proliferation by mediating HDAC2 and GLI2 expression. Cancer Science, n/a(n/a). https://doi.org/https://doi.org/10.1111/cas.14868 Cite
