Effects of intestinal epithelial cells transfected with metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) lentiviruses and Smart Silencer, on monolayer permeability and apical junction complex proteins were analyzed.
[Journal of Crohns & Colitis]
6445212
{6445212:HESTGLKH}
apa
50
1
160044
https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-bf474c70f093a7ff931cfd303cfcc575%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22HESTGLKH%22%2C%22library%22%3A%7B%22id%22%3A6445212%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Li%20et%20al.%22%2C%22parsedDate%22%3A%222021-03-02%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ELi%2C%20Y.%2C%20Zhu%2C%20L.%2C%20Chen%2C%20P.%2C%20Wang%2C%20Y.%2C%20Yang%2C%20G.%2C%20Zhou%2C%20G.%2C%20Li%2C%20L.%2C%20Feng%2C%20R.%2C%20Qiu%2C%20Y.%2C%20Han%2C%20J.%2C%20Chen%2C%20B.%2C%20He%2C%20Y.%2C%20Zeng%2C%20Z.%2C%20Chen%2C%20M.%2C%20%26amp%3B%20Zhang%2C%20S.%20%282021%29.%20MALAT1%20maintains%20the%20intestinal%20mucosal%20homeostasis%20in%20Crohn%26%23x2019%3Bs%20disease%20via%20the%20miR-146b-5p-CLDN11%5C%2FNUMB%20pathway.%20%3Ci%3EJournal%20of%20Crohn%26%23x2019%3Bs%20and%20Colitis%3C%5C%2Fi%3E%2C%20%3Ci%3Ejjab040%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fecco-jcc%5C%2Fjjab040%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fecco-jcc%5C%2Fjjab040%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445212%26amp%3Bitem_key%3DHESTGLKH%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22MALAT1%20maintains%20the%20intestinal%20mucosal%20homeostasis%20in%20Crohn%5Cu2019s%20disease%20via%20the%20miR-146b-5p-CLDN11%5C%2FNUMB%20pathway%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ying%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Liguo%22%2C%22lastName%22%3A%22Zhu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Peng%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ying%22%2C%22lastName%22%3A%22Wang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Guang%22%2C%22lastName%22%3A%22Yang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gaoshi%22%2C%22lastName%22%3A%22Zhou%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Li%22%2C%22lastName%22%3A%22Li%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rui%22%2C%22lastName%22%3A%22Feng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yun%22%2C%22lastName%22%3A%22Qiu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jing%22%2C%22lastName%22%3A%22Han%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Baili%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yao%22%2C%22lastName%22%3A%22He%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhirong%22%2C%22lastName%22%3A%22Zeng%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Minhu%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Shenghong%22%2C%22lastName%22%3A%22Zhang%22%7D%5D%2C%22abstractNote%22%3A%22Intestinal%20homeostasis%20disorder%20is%20critical%20for%20developing%20Crohn%27s%20disease%20%28CD%29.%20Maintaining%20mucosal%20barrier%20integrity%20is%20essential%20for%20intestinal%20homeostasis%2C%20preventing%20intestinal%20injury%20and%20complications.%20Among%20the%20remarkably%20altered%20long%20non-coding%20RNAs%20%28lncRNAs%29%20in%20CD%2C%20we%20aimed%20to%20investigate%20whether%20metastasis-associated%20lung%20adenocarcinoma%20transcript%201%20%28MALAT1%29%20modulated%20CD%20and%20consequent%20disruption%20of%20intestinal%20homeostasis.Microarray%20analyses%20on%20intestinal%20mucosa%20of%20CD%20patients%20and%20controls%20were%20performed%20to%20identify%20dysregulated%20lncRNAs.%20MALAT1%20expression%20was%20investigated%20via%20qRT-PCR%20and%20its%20distribution%20in%20intestinal%20tissues%20was%20detected%20using%20BaseScope.%20Intestines%20from%20MALAT1%20knockout%20mice%20with%20colitis%20were%20investigated%20using%20histological%2C%20molecular%20and%20biochemical%20approaches.%20Effects%20of%20intestinal%20epithelial%20cells%20transfected%20with%20MALAT1%20lentiviruses%20and%20Smart%20Silencer%2C%20on%20monolayer%20permeability%20and%20apical%20junction%20complex%20%28AJC%29%20proteins%20were%20analysed.%20MiR-146b-5p%20were%20confirmed%20as%20a%20critical%20MALAT1%20mediator%20in%20cells%20transfected%20with%20miR-146b-5p%20mimic%5C%2Finhibitor%20and%20in%20colitis%20mice%20administered%20with%20agomir-146b-5p%5C%2Fantagomir-146b-5p.%20Interaction%20between%20MALAT1%20and%20miR-146b-5p%20was%20predicted%20via%20bioinformatics%20and%20validated%20using%20Dual-luciferase%20reporter%20assay%20and%20Ago2-RIP.MALAT1%20was%20aberrantly%20downregulated%20in%20the%20intestine%20mucosa%20of%20CD%20patients%20and%20mice%20with%20experimental%20colitis.%20MALAT1%20knockout%20mice%20were%20hypersensitive%20to%20DSS-induced%20experimental%20colitis.%20MALAT1%20regulated%20intestinal%20mucosal%20barrier%20and%20regained%20intestinal%20homeostasis%20by%20sequestering%20miR-146b-5p%20and%20maintaining%20the%20expression%20of%20the%20AJC%20proteins%20NUMB%20and%20CLDN11.Downregulation%20of%20MALAT1%20contributed%20to%20the%20pathogenesis%20of%20CD%20by%20disrupting%20AJC.%20Thus%2C%20a%20specific%20MALAT1-miR-146b-5p-NUMB%5C%2FCLDN11%20pathway%20that%20plays%20a%20vital%20role%20in%20maintaining%20intestinal%20mucosal%20homeostasis%20may%20serve%20as%20a%20novel%20target%20for%20CD%20treatment.%22%2C%22date%22%3A%22March%202%2C%202021%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1093%5C%2Fecco-jcc%5C%2Fjjab040%22%2C%22ISSN%22%3A%221873-9946%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1093%5C%2Fecco-jcc%5C%2Fjjab040%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-03-12T18%3A01%3A35Z%22%7D%7D%5D%7D
Li, Y., Zhu, L., Chen, P., Wang, Y., Yang, G., Zhou, G., Li, L., Feng, R., Qiu, Y., Han, J., Chen, B., He, Y., Zeng, Z., Chen, M., & Zhang, S. (2021). MALAT1 maintains the intestinal mucosal homeostasis in Crohn’s disease via the miR-146b-5p-CLDN11/NUMB pathway. Journal of Crohn’s and Colitis, jjab040. https://doi.org/10.1093/ecco-jcc/jjab040 Cite
