Scientists investigated the effect of 5, 10, or 20% pHPL in culture media on adipose-derived stromal/stem cell proliferation and phenotypic marker expression.
[Cells Tissues Organs]
6445218
{6445218:YKTGS9H2}
apa
50
1
160394
https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-6d01df90a7f3c6b002c5a67e942fcc2d%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22YKTGS9H2%22%2C%22library%22%3A%7B%22id%22%3A6445218%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Ballesteros%20et%20al.%22%2C%22parsedDate%22%3A%222021-03-22%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EBallesteros%2C%20O.%20R.%2C%20Brooks%2C%20P.%20T.%2C%20Haastrup%2C%20E.%20K.%2C%20Fischer-Nielsen%2C%20A.%2C%20Munthe-Fog%2C%20L.%2C%20%26amp%3B%20Svalgaard%2C%20J.%20D.%20%282021%29.%20Adipose-Derived%20Stromal%5C%2FStem%20Cell%20Culture%3A%20Effects%20of%20Different%20Concentrations%20of%20Human%20Platelet%20Lysate%20in%20Media.%20%3Ci%3ECells%20Tissues%20Organs%3C%5C%2Fi%3E%2C%201%26%23x2013%3B9.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1159%5C%2F000513604%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1159%5C%2F000513604%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445218%26amp%3Bitem_key%3DYKTGS9H2%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Adipose-Derived%20Stromal%5C%2FStem%20Cell%20Culture%3A%20Effects%20of%20Different%20Concentrations%20of%20Human%20Platelet%20Lysate%20in%20Media%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olga%20R.%22%2C%22lastName%22%3A%22Ballesteros%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Patrick%20T.%22%2C%22lastName%22%3A%22Brooks%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eva%20K.%22%2C%22lastName%22%3A%22Haastrup%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Anne%22%2C%22lastName%22%3A%22Fischer-Nielsen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lea%22%2C%22lastName%22%3A%22Munthe-Fog%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jesper%20D.%22%2C%22lastName%22%3A%22Svalgaard%22%7D%5D%2C%22abstractNote%22%3A%22Adipose-derived%20stromal%5C%2Fstem%20cells%20%28ASCs%29%20are%20being%20tested%20as%20a%20possible%20treatment%20for%20a%20wide%20range%20of%20diseases%20to%20exploit%20the%20immunomodulatory%20and%20regenerative%20potential%20demonstrated%20in%20vitro.%20Pooled%20human%20platelet%20lysate%20%28pHPL%29%20has%20replaced%20fetal%20bovine%20serum%20%28FBS%29%20as%20the%20preferred%20growth%20supplement%20because%20of%20its%20xeno-free%20origin%20and%20improved%20cell%20proliferation.%20Much%20has%20been%20done%20toward%20reducing%20the%20concentration%20of%20pHPL%20required%20when%20expanding%20ASCs.%20However%2C%20little%20is%20known%20on%20how%20increasing%20the%20concentration%20of%20pHPL%20affects%20ASC%20potency%2C%20which%20could%20lead%20to%20changes%20with%20possible%20beneficial%20applications.%20This%20study%20investigated%20the%20effect%20of%205%2C%2010%2C%20or%2020%25%20pHPL%20in%20culture%20media%20on%20ASC%20proliferation%20and%20phenotypic%20marker%20expression%2C%20including%20chemokine%20receptors%20CXCR2%2C%20CXCR3%2C%20CXCR4%2C%20and%20VLA-4.%20Adipogenic%20and%20osteogenic%20properties%2C%20as%20well%20as%20immunosuppressive%20properties%2C%20including%20the%20ability%20to%20induce%20indoleamine-pyrrole%202%2C3-dioxygenase%201%20%28IDO1%29%20and%20suppress%20T%20cell%20proliferation%2C%20were%20also%20examined.%20We%20observed%20a%20significant%20increase%20in%20cell%20yield%20%28approximately%202-fold%29%20and%20a%20corresponding%20reduction%20in%20population%20doubling%20time%20and%20cell%20volume%20when%20doubling%20the%20concentration%20of%20pHPL%20in%20the%20growth%20media.%20ASCs%20maintained%20expression%20of%20phenotypic%20surface%20markers%20CD73%2C%20CD90%2C%20and%20CD105%20and%20were%20negative%20for%20CD45%20and%20CD31.%20The%20ability%20to%20induce%20IDO1%20and%20suppress%20T%20cell%20proliferation%20was%20observed%20as%20well.%20Adipogenesis%20and%20osteogenesis%2C%20however%2C%20seem%20to%20be%20increased%20at%20higher%20concentrations%20of%20pHPL%20%2820%25%20%26%23x3e%3B%2010%25%20%26%23x3e%3B%205%25%29%2C%20while%20expression%20of%20chemokine%20receptors%20CXCR2%20and%20CXCR3%20was%20lower.%20In%20conclusion%2C%20increasing%20the%20pHPL%20concentration%20to%2020%25%20could%20be%20used%20to%20optimize%20culture%20conditions%20when%20producing%20cells%20for%20clinical%20treatments%20and%20may%20even%20be%20used%20to%20enhance%20beneficial%20ASC%20properties%20depending%20on%20the%20desired%20therapeutic%20effect.%22%2C%22date%22%3A%222021%5C%2F03%5C%2F22%22%2C%22language%22%3A%22english%22%2C%22DOI%22%3A%2210.1159%5C%2F000513604%22%2C%22ISSN%22%3A%221422-6405%2C%201422-6421%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.karger.com%5C%2FArticle%5C%2FFullText%5C%2F513604%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-03-23T19%3A12%3A09Z%22%7D%7D%5D%7D
Ballesteros, O. R., Brooks, P. T., Haastrup, E. K., Fischer-Nielsen, A., Munthe-Fog, L., & Svalgaard, J. D. (2021). Adipose-Derived Stromal/Stem Cell Culture: Effects of Different Concentrations of Human Platelet Lysate in Media. Cells Tissues Organs, 1–9. https://doi.org/10.1159/000513604 Cite
