Transient overexpression of miR-138 in glioblastoma cells inhibited cell proliferation, cell cycle, migration, and wound healing capability.The authors unveiled that miR-138 negatively regulated the expression of CD44 by directly binding to the 3′ UTR of CD44.
[Scientific Reports]
6807162
{6807162:427Y4CP6}
apa
50
1
161634
https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-5850946d862727a21415eda735cb11b8%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22427Y4CP6%22%2C%22library%22%3A%7B%22id%22%3A6807162%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Yeh%20et%20al.%22%2C%22parsedDate%22%3A%222021-04-28%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EYeh%2C%20M.%2C%20Wang%2C%20Y.-Y.%2C%20Yoo%2C%20J.%20Y.%2C%20Oh%2C%20C.%2C%20Otani%2C%20Y.%2C%20Kang%2C%20J.%20M.%2C%20Park%2C%20E.%20S.%2C%20Kim%2C%20E.%2C%20Chung%2C%20S.%2C%20Jeon%2C%20Y.-J.%2C%20Calin%2C%20G.%20A.%2C%20Kaur%2C%20B.%2C%20Zhao%2C%20Z.%2C%20%26amp%3B%20Lee%2C%20T.%20J.%20%282021%29.%20MicroRNA-138%20suppresses%20glioblastoma%20proliferation%20through%20downregulation%20of%20CD44.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%209219.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-88615-8%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-88615-8%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6807162%26amp%3Bitem_key%3D427Y4CP6%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22MicroRNA-138%20suppresses%20glioblastoma%20proliferation%20through%20downregulation%20of%20CD44%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Margaret%22%2C%22lastName%22%3A%22Yeh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yin-Ying%22%2C%22lastName%22%3A%22Wang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ji%20Young%22%2C%22lastName%22%3A%22Yoo%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Christina%22%2C%22lastName%22%3A%22Oh%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yoshihiro%22%2C%22lastName%22%3A%22Otani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jin%20Muk%22%2C%22lastName%22%3A%22Kang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eun%20S.%22%2C%22lastName%22%3A%22Park%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eunhee%22%2C%22lastName%22%3A%22Kim%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Sangwoon%22%2C%22lastName%22%3A%22Chung%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Young-Jun%22%2C%22lastName%22%3A%22Jeon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22George%20A.%22%2C%22lastName%22%3A%22Calin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Balveen%22%2C%22lastName%22%3A%22Kaur%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhongming%22%2C%22lastName%22%3A%22Zhao%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Tae%20Jin%22%2C%22lastName%22%3A%22Lee%22%7D%5D%2C%22abstractNote%22%3A%22Tumor%20suppressive%20microRNAs%20%28miRNAs%29%20are%20increasingly%20implicated%20in%20the%20development%20of%20anti-tumor%20therapy%20by%20reprogramming%20gene%20network%20that%20are%20aberrantly%20regulated%20in%20cancer%20cells.%20This%20study%20aimed%20to%20determine%20the%20therapeutic%20potential%20of%20putative%20tumor%20suppressive%20miRNA%2C%20miR-138%2C%20against%20glioblastoma%20%28GBM%29.%20Whole%20transcriptome%20and%20miRNA%20expression%20profiling%20analyses%20on%20human%20GBM%20patient%20tissues%20identified%20miR-138%20as%20one%20of%20the%20significantly%20downregulated%20miRNAs%20with%20an%20inverse%20correlation%20with%20CD44%20expression.%20Transient%20overexpression%20of%20miR-138%20in%20GBM%20cells%20inhibited%20cell%20proliferation%2C%20cell%20cycle%2C%20migration%2C%20and%20wound%20healing%20capability.%20We%20unveiled%20that%20miR-138%20negatively%20regulates%20the%20expression%20of%20CD44%20by%20directly%20binding%20to%20the%203%5Cu2032%20UTR%20of%20CD44.%20CD44%20inhibition%20by%20miR-138%20resulted%20in%20an%20inhibition%20of%20glioblastoma%20cell%20proliferation%20in%20vitro%20through%20cell%20cycle%20arrest%20as%20evidenced%20by%20a%20significant%20induction%20of%20p27%20and%20its%20translocation%20into%20nucleus.%20Ectopic%20expression%20of%20miR-138%20also%20increased%20survival%20rates%20in%20mice%20that%20had%20an%20intracranial%20xenograft%20tumor%20derived%20from%20human%20patient-derived%20primary%20GBM%20cells.%20In%20conclusion%2C%20we%20demonstrated%20a%20therapeutic%20potential%20of%20tumor%20suppressive%20miR-138%20through%20direct%20downregulation%20of%20CD44%20for%20the%20treatment%20of%20primary%20GBM.%22%2C%22date%22%3A%222021-04-28%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-88615-8%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-021-88615-8%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-04-28T16%3A28%3A29Z%22%7D%7D%5D%7D
Yeh, M., Wang, Y.-Y., Yoo, J. Y., Oh, C., Otani, Y., Kang, J. M., Park, E. S., Kim, E., Chung, S., Jeon, Y.-J., Calin, G. A., Kaur, B., Zhao, Z., & Lee, T. J. (2021). MicroRNA-138 suppresses glioblastoma proliferation through downregulation of CD44. Scientific Reports, 11(1), 9219. https://doi.org/10.1038/s41598-021-88615-8 Cite
