Home Newsletters Intestinal Cell News MiR-205-5p Suppresses Angiogenesis in Gastric Cancer by Downregulating the Expression of VEGFA...

MiR-205-5p Suppresses Angiogenesis in Gastric Cancer by Downregulating the Expression of VEGFA and FGF1

0
To help identify new strategies for antiangiogenic therapy in gastric cancer (GC), scientists evaluated miR-205-5p expression in GC tissues from TCGA database and our hospital, and its functions in angiogenesis were explored in vitro and in vivo.
[Experimental Cell Research]
6445218 {6445218:YBHBVBWQ} apa 50 1 162033 https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-13c50f83aceca4b5c92da46d5bc46c43%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22YBHBVBWQ%22%2C%22library%22%3A%7B%22id%22%3A6445218%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Zhang%20et%20al.%22%2C%22parsedDate%22%3A%222021-05-04%22%2C%22numChildren%22%3A1%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EZhang%2C%20J.%2C%20Zhang%2C%20J.%2C%20Pang%2C%20X.%2C%20Chen%2C%20Z.%2C%20Zhang%2C%20Z.%2C%20Lei%2C%20L.%2C%20Xu%2C%20H.%2C%20Wen%2C%20L.%2C%20Zhu%2C%20J.%2C%20Jiang%2C%20Y.%2C%20Cui%2C%20Y.%2C%20Chen%2C%20G.%2C%20%26amp%3B%20Wang%2C%20X.%20%282021%29.%20MiR-205-5p%20suppresses%20angiogenesis%20in%20gastric%20cancer%20by%20downregulating%20the%20expression%20of%20VEGFA%20and%20FGF1.%20%3Ci%3EExperimental%20Cell%20Research%3C%5C%2Fi%3E%2C%20112579.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.yexcr.2021.112579%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1016%5C%2Fj.yexcr.2021.112579%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445218%26amp%3Bitem_key%3DYBHBVBWQ%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22MiR-205-5p%20suppresses%20angiogenesis%20in%20gastric%20cancer%20by%20downregulating%20the%20expression%20of%20VEGFA%20and%20FGF1%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Junling%22%2C%22lastName%22%3A%22Zhang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jixin%22%2C%22lastName%22%3A%22Zhang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xiaocong%22%2C%22lastName%22%3A%22Pang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ziyi%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Zhuo%22%2C%22lastName%22%3A%22Zhang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Lili%22%2C%22lastName%22%3A%22Lei%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hongliang%22%2C%22lastName%22%3A%22Xu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Long%22%2C%22lastName%22%3A%22Wen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jing%22%2C%22lastName%22%3A%22Zhu%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yong%22%2C%22lastName%22%3A%22Jiang%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yimin%22%2C%22lastName%22%3A%22Cui%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Guowei%22%2C%22lastName%22%3A%22Chen%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Xin%22%2C%22lastName%22%3A%22Wang%22%7D%5D%2C%22abstractNote%22%3A%22Anti-angiogenic%20therapy%20represents%20one%20of%20the%20most%20promising%20treatment%20modalities%20for%20human%20cancers.%20However%2C%20the%20response%20to%20antiangiogenic%20therapy%20in%20gastric%20cancer%20%28GC%29%20remains%20dismal.%20To%20help%20identify%20new%20strategies%20for%20antiangiogenic%20therapy%20in%20GC%2C%20we%20evaluated%20miR-205-5p%20expression%20in%20GC%20tissues%20from%20TCGA%20database%20and%20our%20hospital%2C%20and%20its%20functions%20in%20angiogenesis%20were%20explored%20in%20vitro%20and%20in%20vivo.%20We%20investigated%20miR-205-5p%20expression%20and%20microvessel%20densities%20%28MVDs%29%20in%20GC%20tissues%20and%20liver%20metastases%20from%20patients.%20The%20function%20and%20mechanisms%20of%20miR-205-5p%20were%20examined%20in%20human%20cell%20lines%20and%20in%20xenograft%20mouse%20models.%20Associations%20between%20miR-205-5p%20expression%20and%20clinical%20characteristics%20were%20analyzed%20using%20either%20Pearson%5Cu2019s%20%5Cu03c72%20test%20or%20Fisher%5Cu2019s%20exact%20test.%20Differences%20in%20overall%20survival%20%28OS%29%20distributions%20were%20evaluated%20using%20the%20log-rank%20test.%20Differences%20in%20measurement%20data%20were%20compared%20using%20Student%5Cu2019s%20t-test%20and%20one-way%20ANOVA.%20We%20found%20that%20miR-205-5p%20expression%20was%20downregulated%20in%20GC%20tissues%20and%20was%20negatively%20correlated%20with%20CD31%20expression%20in%20both%20TCGA%20and%20our%20clinical%20samples.%20GC%20cell%20lines%20expressed%20low%20levels%20of%20miR-205-5p%2C%20and%20miR-205-5p%20upregulation%20significantly%20impaired%20the%20proliferation%20and%20angiogenesis%20of%20GC%20cells.%20Moreover%2C%20vascular%20endothelial%20growth%20factor%20A%20%28VEGFA%29%20and%20fibroblast%20growth%20factor%201%20%28FGF1%29%20expression%20and%20activation%20of%20extracellular-related%20kinase%20%28ERK%29%20signaling%20were%20suppressed%20by%20miR-205-5p.%20MiR-205-5p%20inhibition%20promoted%20malignant%20phenotypes%20by%20enhancing%20VEGFA%20and%20FGF1%20expression%2C%20as%20well%20as%20the%20activation%20of%20ERK%20signaling.%20Angiogenesis%20and%20ERK%20signaling%20were%20decreased%20in%20response%20to%20VEGFA%20and%20FGF1%20downregulation%20induced%20by%20miR-205-5p%20overexpression.%20The%20dual-luciferase%20reporter%20assay%20showed%20that%20VEGFA%20and%20FGF1%20were%20direct%20targets%20of%20miR-205-5p.%20Xenograft%20mouse%20models%20revealed%20that%20miR-205-5p%20suppressed%20tumor%20growth%20by%20inhibiting%20neovascularization.%20Altogether%2C%20these%20results%20demonstrate%20that%20miR-205-5p%20suppresses%20angiogenesis%20in%20GC%20by%20attenuating%20the%20expression%20of%20VEGFA%20and%20FGF1%2C%20indicating%20that%20upregulation%20of%20miR-205-5p%20may%20represent%20as%20an%20antiangiogenic%20therapy%20for%20GC.%22%2C%22date%22%3A%22May%204%2C%202021%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1016%5C%2Fj.yexcr.2021.112579%22%2C%22ISSN%22%3A%220014-4827%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.sciencedirect.com%5C%2Fscience%5C%2Farticle%5C%2Fpii%5C%2FS0014482721001117%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-05-07T23%3A17%3A19Z%22%7D%7D%5D%7D
Zhang, J., Zhang, J., Pang, X., Chen, Z., Zhang, Z., Lei, L., Xu, H., Wen, L., Zhu, J., Jiang, Y., Cui, Y., Chen, G., & Wang, X. (2021). MiR-205-5p suppresses angiogenesis in gastric cancer by downregulating the expression of VEGFA and FGF1. Experimental Cell Research, 112579. https://doi.org/10.1016/j.yexcr.2021.112579 Cite
Abstract
Exit mobile version