By analyzing retinal explants treated with selective ligands that target these receptors, we found that Sstr2-dependent somatostatin signaling induces a modest, dose-dependent inhibition of photoreceptor generation, while correspondingly increasing the relative fraction of primary progenitor cells.
[Scientific Reports]
7753456
{7753456:28K4SEA2}
apa
50
1
162643
https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-b24c7734260cd793247d6bc2519e0407%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%2228K4SEA2%22%2C%22library%22%3A%7B%22id%22%3A7753456%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Weir%20et%20al.%22%2C%22parsedDate%22%3A%222021-05-26%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EWeir%2C%20K.%2C%20Kim%2C%20D.%20W.%2C%20%26amp%3B%20Blackshaw%2C%20S.%20%282021%29.%20A%20potential%20role%20for%20somatostatin%20signaling%20in%20regulating%20retinal%20neurogenesis.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%2010962.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-90554-3%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-90554-3%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D7753456%26amp%3Bitem_key%3D28K4SEA2%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22A%20potential%20role%20for%20somatostatin%20signaling%20in%20regulating%20retinal%20neurogenesis%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Kurt%22%2C%22lastName%22%3A%22Weir%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Dong%20Won%22%2C%22lastName%22%3A%22Kim%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Seth%22%2C%22lastName%22%3A%22Blackshaw%22%7D%5D%2C%22abstractNote%22%3A%22Neuropeptides%20have%20been%20reported%20to%20regulate%20progenitor%20proliferation%20and%20neurogenesis%20in%20the%20central%20nervous%20system.%20However%2C%20these%20studies%20have%20typically%20been%20conducted%20using%20pharmacological%20agents%20in%20ex%20vivo%20preparations%2C%20and%20in%20vivo%20evidence%20for%20their%20developmental%20function%20is%20generally%20lacking.%20Recent%20scRNA-Seq%20studies%20have%20identified%20multiple%20neuropeptides%20and%20their%20receptors%20as%20being%20selectively%20expressed%20in%20neurogenic%20progenitors%20of%20the%20embryonic%20mouse%20and%20human%20retina.%20This%20includes%20Sstr2%2C%20whose%20ligand%20somatostatin%20is%20transiently%20expressed%20by%20immature%20retinal%20ganglion%20cells.%20By%20analyzing%20retinal%20explants%20treated%20with%20selective%20ligands%20that%20target%20these%20receptors%2C%20we%20found%20that%20Sstr2-dependent%20somatostatin%20signaling%20induces%20a%20modest%2C%20dose-dependent%20inhibition%20of%20photoreceptor%20generation%2C%20while%20correspondingly%20increasing%20the%20relative%20fraction%20of%20primary%20progenitor%20cells.%20These%20effects%20were%20confirmed%20by%20scRNA-Seq%20analysis%20of%20retinal%20explants%20but%20abolished%20in%20Sstr2-deficient%20retinas.%20Although%20no%20changes%20in%20the%20relative%20fraction%20of%20primary%20progenitors%20or%20photoreceptor%20precursors%20were%20observed%20in%20Sstr2-deficient%20retinas%20in%20vivo%2C%20scRNA-Seq%20analysis%20demonstrated%20accelerated%20differentiation%20of%20neurogenic%20progenitors.%20We%20conclude%20that%2C%20while%20Sstr2%20signaling%20may%20act%20to%20negatively%20regulate%20retinal%20neurogenesis%20in%20combination%20with%20other%20retinal%20ganglion%20cell-derived%20secreted%20factors%20such%20as%20Shh%2C%20it%20is%20dispensable%20for%20normal%20retinal%20development.%22%2C%22date%22%3A%222021-05-26%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-90554-3%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-021-90554-3%22%2C%22deleted%22%3A1%2C%22collections%22%3A%5B%22TUURQW3H%22%5D%2C%22dateModified%22%3A%222021-05-26T16%3A56%3A54Z%22%7D%7D%5D%7D
Weir, K., Kim, D. W., & Blackshaw, S. (2021). A potential role for somatostatin signaling in regulating retinal neurogenesis. Scientific Reports, 11(1), 10962. https://doi.org/10.1038/s41598-021-90554-3 Cite
