To investigate the effect of OEA, the expression of proinflammatory cytokines was measured by RT-qPCR and ELISA in airway epithelial (A549) cells.
[Experimental and Molecular Medicine]
7753456
{7753456:3X9WVVGQ}
apa
50
1
162965
https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-80695c77146a875bafb767932e342242%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%223X9WVVGQ%22%2C%22library%22%3A%7B%22id%22%3A7753456%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Kwon%20et%20al.%22%2C%22parsedDate%22%3A%222021-06-02%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EKwon%2C%20E.-K.%2C%20Choi%2C%20Y.%2C%20Yoon%2C%20I.-H.%2C%20Won%2C%20H.-K.%2C%20Sim%2C%20S.%2C%20Lee%2C%20H.-R.%2C%20Kim%2C%20H.%20S.%2C%20Ye%2C%20Y.-M.%2C%20Shin%2C%20Y.%20S.%2C%20Park%2C%20H.-S.%2C%20%26amp%3B%20Ban%2C%20G.-Y.%20%282021%29.%20Oleoylethanolamide%20induces%20eosinophilic%20airway%20inflammation%20in%20bronchial%20asthma.%20%3Ci%3EExperimental%20%26amp%3B%20Molecular%20Medicine%3C%5C%2Fi%3E%2C%201%26%23x2013%3B10.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs12276-021-00622-x%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs12276-021-00622-x%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D7753456%26amp%3Bitem_key%3D3X9WVVGQ%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Oleoylethanolamide%20induces%20eosinophilic%20airway%20inflammation%20in%20bronchial%20asthma%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Eun-Kyung%22%2C%22lastName%22%3A%22Kwon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Youngwoo%22%2C%22lastName%22%3A%22Choi%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Il-Hee%22%2C%22lastName%22%3A%22Yoon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ha-Kyeong%22%2C%22lastName%22%3A%22Won%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Soyoon%22%2C%22lastName%22%3A%22Sim%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hee-Ra%22%2C%22lastName%22%3A%22Lee%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hyoung%20Su%22%2C%22lastName%22%3A%22Kim%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Young-Min%22%2C%22lastName%22%3A%22Ye%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Yoo%20Seob%22%2C%22lastName%22%3A%22Shin%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hae-Sim%22%2C%22lastName%22%3A%22Park%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ga-Young%22%2C%22lastName%22%3A%22Ban%22%7D%5D%2C%22abstractNote%22%3A%22Asthma%20is%20a%20chronic%20eosinophilic%20inflammatory%20disease%20with%20an%20increasing%20prevalence%20worldwide.%20Endocannabinoids%20are%20known%20to%20have%20immunomodulatory%20biological%20effects.%20However%2C%20the%20contribution%20of%20oleoylethanolamide%20%28OEA%29%20to%20airway%20inflammation%20remains%20to%20be%20elucidated.%20To%20investigate%20the%20effect%20of%20OEA%2C%20the%20expression%20of%20proinflammatory%20cytokines%20was%20measured%20by%20RT-qPCR%20and%20ELISA%20in%20airway%20epithelial%20%28A549%29%20cells.%20The%20numbers%20of%20airway%20inflammatory%20cells%20and%20cytokine%20levels%20in%20bronchoalveolar%20lavage%20fluid%2C%20airway%20hyperresponsiveness%2C%20and%20type%202%20innate%20lymphoid%20cells%20%28ILC2s%29%20were%20examined%20in%20BALB%5C%2Fc%20mice%20after%204%20days%20of%20OEA%20treatment.%20Furthermore%2C%20eosinophil%20activation%20after%20OEA%20treatment%20was%20evaluated%20by%20measuring%20cellular%20CD69%20levels%20in%20eosinophils%20from%20human%20peripheral%20eosinophils%20using%20flow%20cytometry.%20OEA%20induced%20type%202%20inflammatory%20responses%20in%20vitro%20and%20in%20vivo.%20OEA%20increased%20the%20levels%20of%20proinflammatory%20cytokines%2C%20such%20as%20IL-6%2C%20IL-8%2C%20and%20IL-33%2C%20in%20A549%20cells.%20In%20addition%2C%20it%20also%20induced%20eosinophilic%20inflammation%2C%20the%20production%20of%20IL-4%2C%20IL-5%2C%20IL-13%2C%20and%20IL-33%20in%20bronchoalveolar%20lavage%20fluid%2C%20and%20airway%20hyperresponsiveness.%20OEA%20increased%20the%20numbers%20of%20IL-5-%20or%20IL-13-producing%20ILC2s%20in%20a%20mouse%20model.%20Finally%2C%20we%20confirmed%20that%20OEA%20increased%20CD69%20expression%20%28an%20eosinophil%20activation%20marker%29%20on%20purified%20eosinophils%20from%20patients%20with%20asthma%20compared%20to%20those%20from%20healthy%20controls.%20OEA%20may%20play%20a%20role%20in%20the%20pathogenesis%20of%20asthma%20by%20activating%20ILC2s%20and%20eosinophils.%22%2C%22date%22%3A%222021-06-02%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs12276-021-00622-x%22%2C%22ISSN%22%3A%222092-6413%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs12276-021-00622-x%22%2C%22deleted%22%3A1%2C%22collections%22%3A%5B%2243ZBJFKZ%22%5D%2C%22dateModified%22%3A%222021-06-03T17%3A12%3A04Z%22%7D%7D%5D%7D
Kwon, E.-K., Choi, Y., Yoon, I.-H., Won, H.-K., Sim, S., Lee, H.-R., Kim, H. S., Ye, Y.-M., Shin, Y. S., Park, H.-S., & Ban, G.-Y. (2021). Oleoylethanolamide induces eosinophilic airway inflammation in bronchial asthma. Experimental & Molecular Medicine, 1–10. https://doi.org/10.1038/s12276-021-00622-x Cite
