Scientists used a modular tissue-engineering approach, embedding SUP microgels loaded with human umbilical vein endothelial cells into a 3D SUP hydrogel containing human dermal fibroblast neonatal cells to trigger angiogenesis.
[ACS Applied Materials & Interfaces]
6445218
{6445218:SYMS9XTH}
apa
50
1
163623
https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-96665729e7f683780188ed7e19aa37f4%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%22SYMS9XTH%22%2C%22library%22%3A%7B%22id%22%3A6445218%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Ramirez-Calderon%20et%20al.%22%2C%22parsedDate%22%3A%222021-06-18%22%2C%22numChildren%22%3A0%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3ERamirez-Calderon%2C%20G.%2C%20Susapto%2C%20H.%20H.%2C%20%26amp%3B%20Hauser%2C%20C.%20A.%20E.%20%282021%29.%20Delivery%20of%20Endothelial%20Cell-Laden%20Microgel%20Elicits%20Angiogenesis%20in%20Self-Assembling%20Ultrashort%20Peptide%20Hydrogels%20In%20Vitro.%20%3Ci%3EACS%20Applied%20Materials%20%26amp%3B%20Interfaces%3C%5C%2Fi%3E.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facsami.1c03787%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facsami.1c03787%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445218%26amp%3Bitem_key%3DSYMS9XTH%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Delivery%20of%20Endothelial%20Cell-Laden%20Microgel%20Elicits%20Angiogenesis%20in%20Self-Assembling%20Ultrashort%20Peptide%20Hydrogels%20In%20Vitro%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gustavo%22%2C%22lastName%22%3A%22Ramirez-Calderon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Hepi%20Hari%22%2C%22lastName%22%3A%22Susapto%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Charlotte%20A.%20E.%22%2C%22lastName%22%3A%22Hauser%22%7D%5D%2C%22abstractNote%22%3A%22Blood%20vessel%20generation%20is%20an%20essential%20process%20for%20tissue%20formation%2C%20regeneration%2C%20and%20repair.%20Notwithstanding%2C%20vascularized%20tissue%20fabrication%20in%20vitro%20remains%20a%20challenge%2C%20as%20current%20fabrication%20techniques%20and%20biomaterials%20lack%20translational%20potential%20in%20medicine.%20Naturally%20derived%20biomaterials%20harbor%20the%20risk%20of%20immunogenicity%20and%20pathogen%20transmission%2C%20while%20synthetic%20materials%20need%20functionalization%20or%20blending%20to%20improve%20their%20biocompatibility.%20In%20addition%2C%20the%20traditional%20top-down%20fabrication%20techniques%20do%20not%20recreate%20the%20native%20tissue%20microarchitecture.%20Self-assembling%20ultrashort%20peptides%20%28SUPs%29%20are%20promising%20chemically%20synthesized%20natural%20materials%20that%20self-assemble%20into%20three-dimensional%20nanofibrous%20hydrogels%20resembling%20the%20extracellular%20matrix%20%28ECM%29.%20Here%2C%20we%20use%20a%20modular%20tissue-engineering%20approach%2C%20embedding%20SUP%20microgels%20loaded%20with%20human%20umbilical%20vein%20endothelial%20cells%20%28HUVECs%29%20into%20a%203D%20SUP%20hydrogel%20containing%20human%20dermal%20fibroblast%20neonatal%20%28HDFn%29%20cells%20to%20trigger%20angiogenesis.%20The%20SUPs%20IVFK%20and%20IVZK%20were%20used%20to%20fabricate%20microgels%20that%20gel%20in%20a%20water-in-oil%20emulsion%20using%20a%20microfluidic%20droplet%20generator%20chip.%20The%20fabricated%20SUP%20microgels%20are%20round%20structures%20that%20are%20300%5Cu2013350%20%5Cu03bcm%20diameter%20in%20size%20and%20have%20ECM-like%20topography.%20In%20addition%2C%20they%20are%20stable%20enough%20to%20keep%20their%20original%20size%20and%20shape%20under%20cell%20culture%20conditions%20and%20long-term%20storage.%20When%20the%20SUP%20microgels%20were%20used%20as%20microcarriers%20for%20growing%20HUVECs%20and%20HDFn%20cells%20on%20the%20microgel%20surface%2C%20cell%20attachment%2C%20stretching%2C%20and%20proliferation%20could%20be%20demonstrated.%20Finally%2C%20we%20performed%20an%20angiogenesis%20assay%20in%20both%20SUP%20hydrogels%20using%20all%20SUP%20combinations%20between%20micro-%20and%20bulky%20hydrogels.%20Endothelial%20cells%20were%20able%20to%20migrate%20from%20the%20microgel%20to%20the%20surrounding%20area%2C%20showing%20angiogenesis%20features%20such%20as%20sprouting%2C%20branching%2C%20coalescence%2C%20and%20lumen%20formation.%20Although%20both%20SUP%20hydrogels%20support%20vascular%20network%20formation%2C%20IVFK%20outperformed%20IVZK%20in%20terms%20of%20vessel%20network%20extension%20and%20branching.%20Overall%2C%20these%20results%20demonstrated%20that%20cell-laden%20SUP%20microgels%20have%20great%20potential%20to%20be%20used%20as%20a%20microcarrier%20cell%20delivery%20system%2C%20encouraging%20us%20to%20study%20the%20angiogenesis%20process%20and%20to%20develop%20vascularized%20tissue-engineering%20therapies.%22%2C%22date%22%3A%222021-06-18%22%2C%22language%22%3A%22%22%2C%22DOI%22%3A%2210.1021%5C%2Facsami.1c03787%22%2C%22ISSN%22%3A%221944-8244%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1021%5C%2Facsami.1c03787%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-06-18T21%3A24%3A22Z%22%7D%7D%5D%7D
Ramirez-Calderon, G., Susapto, H. H., & Hauser, C. A. E. (2021). Delivery of Endothelial Cell-Laden Microgel Elicits Angiogenesis in Self-Assembling Ultrashort Peptide Hydrogels In Vitro. ACS Applied Materials & Interfaces. https://doi.org/10.1021/acsami.1c03787 Cite
