The authors aimed at dissecting the cellular and molecular mechanisms driving blood flow-dependent endothelial remodeling. Transcriptomic analysis of endothelial cells revealed that blood flow enhanced VEGFR signaling.
[Scientific Reports]
6445218
{6445218:2MEXPPYN}
apa
50
1
163783
https://www.stemcellsciencenews.com/wp-content/plugins/zotpress/
%7B%22status%22%3A%22success%22%2C%22updateneeded%22%3Afalse%2C%22instance%22%3A%22zotpress-3b40b1dbfce91e9ee46d9cdc22b0adaf%22%2C%22meta%22%3A%7B%22request_last%22%3A0%2C%22request_next%22%3A0%2C%22used_cache%22%3Atrue%7D%2C%22data%22%3A%5B%7B%22key%22%3A%222MEXPPYN%22%2C%22library%22%3A%7B%22id%22%3A6445218%7D%2C%22meta%22%3A%7B%22creatorSummary%22%3A%22Follain%20et%20al.%22%2C%22parsedDate%22%3A%222021-06-23%22%2C%22numChildren%22%3A2%7D%2C%22bib%22%3A%22%3Cdiv%20class%3D%5C%22csl-bib-body%5C%22%20style%3D%5C%22line-height%3A%202%3B%20padding-left%3A%201em%3B%20text-indent%3A-1em%3B%5C%22%3E%5Cn%20%20%3Cdiv%20class%3D%5C%22csl-entry%5C%22%3EFollain%2C%20G.%2C%20Osmani%2C%20N.%2C%20Gensbittel%2C%20V.%2C%20Asokan%2C%20N.%2C%20Larnicol%2C%20A.%2C%20Mercier%2C%20L.%2C%20Garcia-Leon%2C%20M.%20J.%2C%20Busnelli%2C%20I.%2C%20Pichot%2C%20A.%2C%20Paul%2C%20N.%2C%20Carapito%2C%20R.%2C%20Bahram%2C%20S.%2C%20Lefebvre%2C%20O.%2C%20%26amp%3B%20Goetz%2C%20J.%20G.%20%282021%29.%20Impairing%20flow-mediated%20endothelial%20remodeling%20reduces%20extravasation%20of%20tumor%20cells.%20%3Ci%3EScientific%20Reports%3C%5C%2Fi%3E%2C%20%3Ci%3E11%3C%5C%2Fi%3E%281%29%2C%2013144.%20%3Ca%20href%3D%27https%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-92515-2%27%3Ehttps%3A%5C%2F%5C%2Fdoi.org%5C%2F10.1038%5C%2Fs41598-021-92515-2%3C%5C%2Fa%3E%20%3Ca%20title%3D%27Cite%20in%20RIS%20Format%27%20class%3D%27zp-CiteRIS%27%20href%3D%27https%3A%5C%2F%5C%2Fwww.stemcellsciencenews.com%5C%2Fwp-content%5C%2Fplugins%5C%2Fzotpress%5C%2Flib%5C%2Frequest%5C%2Frequest.cite.php%3Fapi_user_id%3D6445218%26amp%3Bitem_key%3D2MEXPPYN%27%3ECite%3C%5C%2Fa%3E%20%3C%5C%2Fdiv%3E%5Cn%3C%5C%2Fdiv%3E%22%2C%22data%22%3A%7B%22itemType%22%3A%22journalArticle%22%2C%22title%22%3A%22Impairing%20flow-mediated%20endothelial%20remodeling%20reduces%20extravasation%20of%20tumor%20cells%22%2C%22creators%22%3A%5B%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Gautier%22%2C%22lastName%22%3A%22Follain%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Na%5Cu00ebl%22%2C%22lastName%22%3A%22Osmani%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Valentin%22%2C%22lastName%22%3A%22Gensbittel%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nandini%22%2C%22lastName%22%3A%22Asokan%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Annabel%22%2C%22lastName%22%3A%22Larnicol%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Luc%22%2C%22lastName%22%3A%22Mercier%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Maria%20Jesus%22%2C%22lastName%22%3A%22Garcia-Leon%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Ignacio%22%2C%22lastName%22%3A%22Busnelli%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Angelique%22%2C%22lastName%22%3A%22Pichot%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Nicod%5Cu00e8me%22%2C%22lastName%22%3A%22Paul%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Rapha%5Cu00ebl%22%2C%22lastName%22%3A%22Carapito%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Seiamak%22%2C%22lastName%22%3A%22Bahram%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Olivier%22%2C%22lastName%22%3A%22Lefebvre%22%7D%2C%7B%22creatorType%22%3A%22author%22%2C%22firstName%22%3A%22Jacky%20G.%22%2C%22lastName%22%3A%22Goetz%22%7D%5D%2C%22abstractNote%22%3A%22Tumor%20progression%20and%20metastatic%20dissemination%20are%20driven%20by%20cell-intrinsic%20and%20biomechanical%20cues%20that%20favor%20the%20growth%20of%20life-threatening%20secondary%20tumors.%20We%20recently%20identified%20pro-metastatic%20vascular%20regions%20with%20blood%20flow%20profiles%20that%20are%20permissive%20for%20the%20arrest%20of%20circulating%20tumor%20cells.%20We%20have%20further%20established%20that%20such%20flow%20profiles%20also%20control%20endothelial%20remodeling%2C%20which%20favors%20extravasation%20of%20arrested%20CTCs.%20Yet%2C%20how%20shear%20forces%20control%20endothelial%20remodeling%20is%20unknown.%20In%20the%20present%20work%2C%20we%20aimed%20at%20dissecting%20the%20cellular%20and%20molecular%20mechanisms%20driving%20blood%20flow-dependent%20endothelial%20remodeling.%20Transcriptomic%20analysis%20of%20endothelial%20cells%20revealed%20that%20blood%20flow%20enhanced%20VEGFR%20signaling%2C%20among%20others.%20Using%20a%20combination%20of%20in%20vitro%20microfluidics%20and%20intravital%20imaging%20in%20zebrafish%20embryos%2C%20we%20now%20demonstrate%20that%20the%20early%20flow-driven%20endothelial%20response%20can%20be%20prevented%20upon%20specific%20inhibition%20of%20VEGFR%20tyrosine%20kinase%20and%20subsequent%20signaling.%20Inhibitory%20targeting%20of%20VEGFRs%20reduced%20endothelial%20remodeling%20and%20subsequent%20metastatic%20extravasation.%20These%20results%20confirm%20the%20importance%20of%20VEGFR-dependent%20endothelial%20remodeling%20as%20a%20driving%20force%20of%20CTC%20extravasation%20and%20metastatic%20dissemination.%20Furthermore%2C%20the%20present%20work%20suggests%20that%20therapies%20targeting%20endothelial%20remodeling%20might%20be%20a%20relevant%20clinical%20strategy%20in%20order%20to%20impede%20metastatic%20progression.%22%2C%22date%22%3A%222021-06-23%22%2C%22language%22%3A%22en%22%2C%22DOI%22%3A%2210.1038%5C%2Fs41598-021-92515-2%22%2C%22ISSN%22%3A%222045-2322%22%2C%22url%22%3A%22https%3A%5C%2F%5C%2Fwww.nature.com%5C%2Farticles%5C%2Fs41598-021-92515-2%22%2C%22collections%22%3A%5B%5D%2C%22dateModified%22%3A%222021-06-23T18%3A24%3A02Z%22%7D%7D%5D%7D
Follain, G., Osmani, N., Gensbittel, V., Asokan, N., Larnicol, A., Mercier, L., Garcia-Leon, M. J., Busnelli, I., Pichot, A., Paul, N., Carapito, R., Bahram, S., Lefebvre, O., & Goetz, J. G. (2021). Impairing flow-mediated endothelial remodeling reduces extravasation of tumor cells. Scientific Reports, 11(1), 13144. https://doi.org/10.1038/s41598-021-92515-2 Cite
