A porcine endothelial cell was characterized and immortalized for genetic modification. Five genes including GGTA1, CMAH, β4galNT2, SLA-I α chain, and β2-microglobulin that are responsible for the production of major xenoantigens were sequentially disrupted in immortalized porcine endothelial cells using CRISPR/Cas9 technology.
[Scientific Reports]
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Li, P., Walsh, J. R., Lopez, K., Isidan, A., Zhang, W., Chen, A. M., Goggins, W. C., Higgins, N. G., Liu, J., Brutkiewicz, R. R., Smith, L. J., Hara, H., Cooper, D. K. C., & Ekser, B. (2021). Genetic engineering of porcine endothelial cell lines for evaluation of human-to-pig xenoreactive immune responses. Scientific Reports, 11(1), 13131. https://doi.org/10.1038/s41598-021-92543-y Cite
