To design CARs with a lower activation threshold, scientists compared antigen-induced pathway signaling in human T cells expressing a current CAR construct to that in cells with endogenous TCRs.
[Science Signaling]
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Salter, A. I., Rajan, A., Kennedy, J. J., Ivey, R. G., Shelby, S. A., Leung, I., Templeton, M. L., Muhunthan, V., Voillet, V., Sommermeyer, D., Whiteaker, J. R., Gottardo, R., Veatch, S. L., Paulovich, A. G., & Riddell, S. R. (2021). Comparative analysis of TCR and CAR signaling informs CAR designs with superior antigen sensitivity and in vivo function. Science Signaling, 14(697). https://doi.org/10.1126/scisignal.abe2606 Cite
