Robust Genome Editing in Adult Vascular Endothelium by Nanoparticle Delivery of CRISPR-Cas9 Plasmid DNA

A single intravenous administration of mixture of nanoparticles and plasmid DNA expressing Cas9 controlled by CDH5 promoter and guide RNA induced highly efficient genome editing in endothelial cells of the vasculatures, including lung, heart, aorta, and peripheral vessels in adult mice.