NewslettersESC & iPSC News Excess of Guide RNA Reduces Knockin Efficiency and Drastically Increases On-Target Large Deletions By Noshin Noorjahan - March 15, 2023 0 Researchers used nano differential scanning fluorimetry, a label and immobilization-free assay, to demonstrate that an equimolar ratio of Cas9 and guide RNA was optimal. [iScience] AbstractGraphical Abstract